التفاصيل البيبلوغرافية
| العنوان: |
Some Endocrine Traits of Transgenic Rabbits. I. Changes in Plasma and Milk Hormones. |
| المؤلفون: |
Sirotkin, A. V., Chrenek, P., Rafay, J., Omelka, R., Vetr, H., Jurčík, R., Binder, B. R. |
| المصدر: |
Physiological Research; 2008, Vol. 57 Issue 5, p735-743, 9p, 5 Graphs |
| مصطلحات موضوعية: |
PHYSIOLOGICAL research, TRANSGENIC organisms, PROGESTERONE, TESTOSTERONE, ESTRADIOL, CORTICOSTERONE |
| مستخلص: |
The aim of these studies was to compare some endocrine and non-endocrine characteristics of transgenic (carrying mammary gland-specific mWAP-hFVIII gene construct) and non-transgenic rabbits. The concentrations of corticosterone, progesterone, testosterone, estradiol, insulin-like growth factor I (IGF-I) and human factor VIII (hFVIII) in the blood plasma of adult females (9 months of age, 3rd generation transgenic animals), adult males, and young females (1-2 months of age, 4th generation of transgenic animals), as well as in the milk of lactating adult females, were analyzed by using RIA. In addition, litter size and body mass of pups born by transgenic and non-transgenic females from the 3rd generation were compared. Transgenic animals were compared with their non-transgenic siblings (the same genetic and epigenetic background). Transgenesis did not influence plasma hFVIII, but significantly increased corticosterone (in all animals), reduced IGF-I (in adult males and females), testosterone and estradiol, (in young females) and altered progesterone (increase in adult males and decrease in adult females) concentrations in blood plasma. In addition, transgenic females had higher milk concentrations of testosterone, but not progesterone or IGF-I than their non-transgenic sisters. These endocrine changes were not associated with changes in litter size. Transgenic male (but not female) pups have smaller body mass than control animals. These observations demonstrate the influence of transgenesis per se on the animal growth and endocrine system (secretion of reproductive and stress steroid hormones as well as growth factors) over four generations. [ABSTRACT FROM AUTHOR] |
|
Copyright of Physiological Research is the property of Institute of Physiology, Academy of Sciences of the Czech Republic and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Supplemental Index |
Full Text Finder