التفاصيل البيبلوغرافية
| العنوان: |
Bacterias fibrolíticas aisladas de rumen de alpaca, ovino y vacuno con capacidad biodegradadora de celulosa. (Spanish) |
| Alternate Title: |
Fibrolytic bacteria isolated from the rumen of alpaca, sheep and cattle with cellulose biodegrading capacity. (English) |
| المؤلفون: |
Carhuapoma-Delacruz, Víctor, Auqui-Acharte, Gissel Shian, Valencia-Mamani, Nicasio, Jesús Gonzales-Huamán, Teresa, Marcelo Guillen-Domínguez, Héctor, Esparza, Mario |
| المصدر: |
Revista Cientifica de la Facultade de Veterinaria; 2022, Vol. 32, p1-7, 7p |
| Abstract (English): |
Cellulolytic ruminal bacteria are used in ruminant feeding due to their biodegradable capacity in fibrous forages. However, there are few studies in alpaca, sheep and cattle whether they constitute sources of cellulose degrading microorganisms for applications in food biotechnology. In this research, the in vitro degradation potential of cellulose of alpaca, sheep and cattle cellulite ruminal bacteria was isolated and evaluated. Ruminal fluid samples were collected from eight alpacas, cattle and sheep specimens from the Municipal slaughterhouse in the town of Huancavelica - Peru, located at 3820 meters above sea level in Peru. Ruminal samples were cultured in media with carboxymethylcellulose, enriched with brain heart infusion broth under aerobic and anaerobic conditions until the development of bacterial colonies was achieved. Then the microbiological, biochemical characterization and cellulase production analysis of each bacterial isolate was performed using the Congo red staining method and the diameter of the halos (greater than 10-14 millimeters (mm)) of cellulose degradability was evaluated. Cellulolytic bacteria Ruminococcus flavefaciens, Ruminococcus albus and Fibrobacter succinogenes with high capacity for cellulose degradability (halo greater than 14 mm) were found. This finding indicates that alpaca, cattle and sheep ruminal fluids are excellent sources of cellulase-producing bacteria with high cellulose degrading capacity. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): |
Las bacterias ruminales celulolíticas se utilizan en la alimentación de rumiantes por su capacidad biodegradable de forrajes fibrosos. Sin embargo, existen escasos estudios en alpaca, ovino y vacuno si constituyen fuentes de microrganismos degradadores de celulosa para aplicaciones en biotecnología alimentaria. En esta investigación se aisló y evaluó el potencial degradador in vitro de la celulosa de las bacterias ruminales celulíticas de alpaca, ovino y vacuno. Se recolectaron muestras de líquido ruminal de ochos especímenes de alpaca, vacuno y ovino del matadero municipal de la localidad de Huancavelica – Perú, ubicado a 3820 metros sobre el nivel del mar en Perú. Las muestras ruminales fueron cultivadas en medios con carboximetilcelulosa, enriquecidos con caldo infusión cerebro corazón en condiciones aeróbicas y anaeróbicas hasta lograr desarrollo de colonias bacterianas. Luego se realizó la caracterización microbiológica, bioquímica y análisis de producción de celulasas de cada aislado bacteriano usando el método de coloración de rojo Congo y se evaluó el diámetro de los halos (mayor a 10-14 milímetros (mm)) de degradabilidad de celulosa. Se encontró bacterias celulolíticas Ruminococcus flavefaciens, Ruminococcus albus y Fibrobacter succinogenes con alta capacidad de degradabilidad de celulosa (halo mayor a 14 mm). Este hallazgo indica que los líquidos ruminales de alpaca, vacuno y ovino son excelentes fuentes de bacterias productoras de celulasas con alta capacidad degradadora de celulosa. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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