التفاصيل البيبلوغرافية
| العنوان: |
Genetic diversity of Mycobacterium tuberculosis isoniazid monoresistant and multidrug-resistant in Rio Grande do Sul, a tuberculosis high-burden state in Brazil. |
| المؤلفون: |
Esteves, Leonardo Souza, Dalla Costa, Elis Regina, Vasconcellos, Sidra Ezidio Gonçalves, Vargas, Andrei, Ferreira Junior, Sérgio Luis Montego, Halon, Maria Laura, Ribeiro, Marta Osorio, Rodenbusch, Rodrigo, Gomes, Harrison Magdinier, Suffys, Philip N., Rossetti, Maria Lucia R. |
| المصدر: |
Tuberculosis (14729792); May2018, Vol. 110, p36-43, 8p |
| مستخلص: |
Tuberculosis (TB) remains a major public health problem in the world and Brazil is among the countries with the highest incidence and prevalence rates, and Rio Grande do Sul, a Brazilian state, occupy a prominent position. Multidrug-resistant Mycobacterium tuberculosis (MDR-TB) further aggravates this scenario, making it more difficult to treat and control the disease. Isoniazid monoresistance (IMR) may increase the risk of progression to MDR-TB and treatment failure. However, most drug resistance molecular tests only focus on detecting rifampicin (RIF) resistance.In the present study, we characterized a total of 63 drug resistant isolates of M. tuberculosis (35 MDR, 26 IMR and two isolates monoresistant to rifampicin [RMR]) of the Rio Grande do Sul state by MIRU-VNTR (24 loci ), spoligotyping, presence of RD Rio , fbp C 103 , pks 15/1 and sequencing of the kat G, rpo B and inh A genes. We observed a higher proportion of the LAM family 30/63 (47.61%). In IMR, mutations were found in the kat G gene (98% at codon 315) in 72.5%, and mutations in the promoter region of the inh A gene in 6.25% of the isolates. In MDR-TB and RMR-TB isolates, 92.1% had mutations in the rpo B gene (57% at codon 531). The presence of a 12 bp insertion between codons 516 and 517 of the rpo B gene in MDR-TB isolates was found in five isolates. In conclusion, we observed that the highest frequency of IMR-TB and MDR-TB strains belong to the LAM and Haarlem genotypes in Rio Grande do Sul state. A significant number of isolates previously characterized as Mycobacterium pinnipedi2 through spoligotyping were found to belong to the M. tuberculosis LAM family. This was responsible for a number of significant cases and the molecular profile of this strain and the pattern of mutations related to drug resistance were analyzed. These findings may contribute to a better understanding about the spread of M. tuberculosis resistant in southern of Brazil. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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