التفاصيل البيبلوغرافية
| العنوان: |
Properdin has an ascendancy over factor H regulation in complement-mediated renal tubular damage. |
| المؤلفون: |
Seiji Nagamachi, Isao Ohsawa, Hiyori Suzuki, Nobuyuki Sato, Hiroyuki Inoshita, Atsuko Hisada, Daisuke Honda, Mamiko Shimamoto, Yoshio Shimizu, Satoshi Horikoshi, Yasuhiko Tomino |
| المصدر: |
BMC Nephrology; 2014, Vol. 15 Issue 1, p1-23, 23p |
| مصطلحات موضوعية: |
PROPERDIN factor B, PROTEINURIA, KIDNEY diseases, KIDNEY tubules, EPITHELIAL cells, MULTIVARIATE analysis, PROTEIN expression, DISEASES |
| مستخلص: |
Background Urinary (U)-complement components have been detected in patients with proteinuric renal diseases, and complement activation via the alternative pathway (AP) is believed to play a role in renal tubular damage. The present study aimed to examine the regulation of complement AP activation in patients with renal tubular damage by focusing on the balance between properdin (P) and factor H (fH). Methods In the in vivo studies, U concentrations of P, fH and membrane attack complex (MAC) were measured in patients with renal diseases using an enzyme-linked immunosorbent assay (ELISA), and their relationships with the clinical data were evaluated. In the in vitro studies, human proximal tubular epithelial cells (PTECs) were incubated with normal human serum (NHS), P-depleted serum (PDS), purified P and/or fH. Changes in cell morphology and phenotype were assessed by microscopy, real-time polymerase chain reaction (PCR), immunostaining and a cell viability assay. Results The U-P, fH and MAC concentrations were significantly higher in patients with renal disease than in normal controls and correlated with the U-protein and tubular damage markers. Furthermore, multivariate analysis revealed a relationship between P levels and tubular damage markers. There were no significant changes in morphology and mRNA expression in the AP components (P, fH, fB, C3, C5 and C9) after the addition of up to 25% NHS. Dosedependent depositions of P or fH were observed after the addition of P or fH on PTECs. Depositions of P were not inhibited by fH in a mixture of a fixed concentration of P and a variable concentration of fH, and vice versa. Preincubation with the fixed concentration of P before the addition of NHS or PDS increased the depositions of P, C3 and MAC compared with incubation with intact NHS or intact PDS only; the depositions of C3 and MAC showed a serum-dependent trend. Preincubation with P before NHS addition significantly suppressed cell viability without causing morphological changes. Conclusions In the pathogenesis of renal tubular damage, P can directly bind to PTECs and may accelerate AP activation by surpassing fH regulation. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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