التفاصيل البيبلوغرافية
| العنوان: |
Effectiveness of two intracanal dressings in adult Portuguese patients: a q PCR and anaerobic culture assessment. |
| المؤلفون: |
Teles, A. M., Manso, M. C., Loureiro, S., Silva, R., Madeira, I. G. C., Pina, C., Cabeda, J. M. |
| المصدر: |
International Endodontic Journal; Jan2014, Vol. 47 Issue 1, p32-40, 9p |
| مصطلحات موضوعية: |
ROOT canal treatment, DENTAL pulp cavities, PERIODONTAL dressings, PORTUGUESE people, POLYMERASE chain reaction, ANAEROBIC infections, DISEASES in adults, THERAPEUTICS, DISEASES |
| مستخلص: |
Aim To quantify bacterial equivalents before and after chemomechanical preparation using 3% sodium hypochlorite ( NaOCl) and intracanal dressing with calcium hydroxide paste ( Ca(OH)2) or 2% Chlorhexidine digluconate gel ( CHX) in necrotic pulps associated or not with apical periodontitis and to further compare this quantification with counts of anaerobic microorganisms. Methodology Prospective clinical trial in 69 single-rooted adult teeth (strict inclusion criteria); CHX group: 34; Ca(OH)2 group: 35. Bacteria samples were taken at baseline ( S1), after chemomechanical preparation ( S2) and after 14 days of intracanal dressing ( S3). Bacterial equivalents were assessed by broad-range real-time polymerase chain reaction (q PCR), and live viable bacteria measured with conventional anaerobic culture ( CFU/mL). Descriptive/inferential analysis was performed with spss vs. 20.0 (α = 0.05) using the Kruskal-Wallis, Mann-Whitney and chi-squared tests and Spearman's correlation coefficients. Results Both groups showed a significant decrease between S1 and S2 (Mann-Whitney U-test; P < 0.001) both in q PCR and in culture. In the Ca(OH)2-group, no variation was observed between S2 and S3 by q PCR and culture. In contrast, the CHX group showed a significant increase from S2 to S3 by both techniques. The two groups were only significantly different in S3 (Mann-Whitney U-test; P ≤ 0.001), with a worse performance in the CHX group. Again, these results were congruent by both approaches. Data from both approaches correlate reasonably ( rS < 0.5). Conclusions Infected root canals contained a high bacterial load, and the chemomechanical root canal preparation reduced bacterial equivalents by 99.1% and anaerobic counts by 98.5%. Intracanal dressings were not efficient at reducing bacterial load, but the 14-day intracanal dressing with Ca(OH)2 performed significantly better than CHX, particularly in cases with apical periodontitis. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |