التفاصيل البيبلوغرافية
| العنوان: |
The SNP at -592 of human IL-10 gene is associated with serum IL-10 levels and increased risk for human papillomavirus cervical lesion development. |
| المؤلفون: |
Torres-Poveda, Kirvis, Burguete-García, Ana I., Cruz, Miguel, Martínez-Nava, Gabriela A., Bahena-Román, Margarita, Ortíz-Flores, Esmeralda, Ramírez-González, Abrahan, López-Estrada, Guillermina, Delgado-Romero, Karina, Madrid-Marina, Vicente |
| المصدر: |
Infectious Agents & Cancer; 2012, Vol. 7 Issue 1, p32-40, 9p, 4 Charts, 2 Graphs |
| مصطلحات موضوعية: |
CERVIX uteri diseases, BIOMARKERS, CHI-squared test, GENES, GENETIC polymorphisms, INTERLEUKINS, INTERVIEWING, RNA, STATISTICS, DATA analysis, MULTIPLE regression analysis, CROSS-sectional method, DISEASE risk factors |
| مصطلحات جغرافية: |
MEXICO |
| مستخلص: |
Background: Women with Human Papilloma Virus (HPV) persistence are characterized by high levels of IL-10 at cervix. We have determined whether polymorphisms of IL-10 gene promoter might be associated with increased risk of squamous intraepithelial cervical lesions (SICL) and whether exist significative differences of IL-10 mRNA expression at cervix and systemic and serum IL-10 protein between SICL cases and non-Cervical Lesions (NCL). Methods: Peripheral blood samples from SICL (n = 204) and NCL (n = 166) were used to detect IL-10 promoter polymorphisms at loci -592A/C (rs1800872), -819C/T (rs1800871), -1082A/G (rs1800896), -1352A/G (rs1800893), by allelic discrimination and to evaluate serum IL-10 protein. Cervical epithelial scrapings from NCL and biopsies from SICLs were used for HPV-typing and to evaluate IL-10 mRNA expression level. The systemic and local IL-10 mRNA expression levels were measured by real time-PCR. Genotypic and allelic frequencies of the selected polymorphisms were analyzed by logistic regression, adjusting by age and HPV-genotype, to determine the association with SICL. Results: No significant differences were found between genotype frequencies at loci -819, -1082, and -1352. Individuals carrying at least one copy of risk allele A of polymorphism -592 had a two-fold increased risk of developing SICL [adjusted odds ratio (OR), 2.02 (95% CI, 1.26-3.25), p = 0.003], compared to NCL. The IL-10 mRNA expression and serum IL-10 protein, were significantly higher in SICL cases (p < 0.01), being higher in patients carrying the risk allele A. Conclusions: The -592 polymorphism is associated with increased risk of SICL and can serve as a marker of genetic susceptibility to SICL among Mexican women. According to IL-10 levels found in SICL, IL-10 can be relevant factor for viral persistence and progression disease. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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