التفاصيل البيبلوغرافية
| العنوان: |
Genetic Characterization of Escherichia coli O157:H7 Strains Isolated from the One-Humped Camel (Camelus dromedarius) by Using Microarray DNA Technology. |
| المؤلفون: |
Salehi, Taghi, Tonelli, Alfreda, Mazza, Alberto, Staji, Hamid, Badagliacca, Pietro, Tamai, Iradj, Jamshidi, Reza, Harel, Josée, Lelli, Rossella, Masson, Luke |
| المصدر: |
Molecular Biotechnology; Jul2012, Vol. 51 Issue 3, p283-288, 6p |
| مستخلص: |
From the Camelidae family members, several serotypes of Escherichia coli ( E. coli) have recently been isolated from diarrhoeic and non-diarrhoeic faecal samples. To date Shiga toxin-producing E. coli (STEC) strains have never been typed in one-humped camel ( Camelus dromedarius). In the present study, two E. coli O157:H7 strains isolated from sick dromedaries were investigated. Virulence gene profiles were determined using a custom E. coli virulence DNA microarray, composed of 70-mer oligonucleotide probes targeting 264 virulence or related genes of known E. coli pathotypes. Both strains displayed positive hybridization signals for the Locus of enterocyte effacement (LEE) gene probes ( ler, eae, espA, espB, tir genes), two Shiga toxin probes ( stx1 and stx2), the O157 O-antigen specific probe, various virulence plasmid ( pO157) probes like katP in addition to other accessory virulence genes characterized in STEC. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |