التفاصيل البيبلوغرافية
| العنوان: |
Peroxicretion: a novel secretion pathway in the eukaryotic cell. |
| المؤلفون: |
Sagt, Cees M. J., ten Haaft, Peter J., Minneboo, Ingeborg M., Hartog, Miranda P., Damveld, Robbert A., van der Laan, Jan Metske, Akeroyd, Michiel, Wenzel, Thibaut J., Luesken, Francisca A., Veenhuis, Marten, van der Klei, Ida, de Winde, Johannes H. |
| المصدر: |
BMC Biotechnology; 2009, Vol. 9, Special section p1-11, 11p, 6 Color Photographs, 3 Black and White Photographs, 2 Diagrams, 2 Graphs |
| مصطلحات موضوعية: |
ENZYMES, CELLS, BACTERIAL genetics, PROTEINS, PEROXISOMES |
| مستخلص: |
Background: Enzyme production in microbial cells has been limited to secreted enzymes or intracellular enzymes followed by expensive down stream processing. Extracellular enzymes consists mainly of hydrolases while intracellular enzymes exhibit a much broader diversity. If these intracellular enzymes could be secreted by the cell the potential of industrial applications of enzymes would be enlarged. Therefore a novel secretion pathway for intracellular proteins was developed, using peroxisomes as secretion vesicles. Results: Peroxisomes were decorated with a Golgi derived v-SNARE using a peroxisomal membrane protein as an anchor. This allowed the peroxisomes to fuse with the plasma membrane. Intracellular proteins were transported into the peroxisomes by adding a peroxisomal import signal (SKL tag). The proteins which were imported in the peroxisomes, were released into the extracellular space through this artificial secretion pathway which was designated peroxicretion. This concept was supported by electron microscopy studies. Conclusion: Our results demonstrate that it is possible to reroute the intracellular trafficking of vesicles by changing the localisation of SNARE molecules, this approach can be used in in vivo biological studies to clarify the different control mechanisms regulating intracellular membrane trafficking. In addition we demonstrate peroxicretion of a diverse set of intracellular proteins. Therefore, we anticipate that the concept of peroxicretion may revolutionize the production of intracellular proteins from fungi and other microbial cells, as well as from mammalian cells. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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