التفاصيل البيبلوغرافية
| العنوان: |
The Orientia tsutsugamushi genome reveals massive proliferation of conjugative type IV secretion system and host—cell interaction genes. |
| المؤلفون: |
Nam-Hyuk Cho, Hang-Rae Kim, Jung-Hee Lee, Se-Yoon Kim, Jaejong Kim, Chat, Sunho, Sang-Yoon Kim, Darby, Alistair C., Fuxelius, Hans-Henrik, Jun Yin, Ju Han Kim, Jihun Kim, Sang Joo Lee, Young-Sang Koh, Won-Jong Jang, Park, Kyung-Hee, Andersson, Siv G. E., Myung-Sik Choi, Ik-Sang Kim |
| المصدر: |
Proceedings of the National Academy of Sciences of the United States of America; 5/8/2007, Vol. 104 Issue 19, p7981-7986, 6p, 2 Diagrams, 2 Graphs |
| مصطلحات موضوعية: |
RICKETTSIA tsutsugamushi, TSUTSUGAMUSHI disease, BACTERIAL genomes, GENOMES, CELL communication, PATHOGENIC microorganisms |
| مستخلص: |
Scrub typhus is caused by the obligate intracellular rickettsia Orientia tsutsugamushi (previously called Rickettsia tsutsugamushi). The bacterium is maternally inherited in trombicuid mites and transmitted to humans by feeding larvae. We report here the 2,127,051-bp genome of the Boryong strain, which represents the most highly repeated bacterial genome sequenced to date. The repeat density of the scrub typhus pathogen is 200-fold higher than that of its close relative Rickettsia prowazekii, the agent of epidemic typhus. A total of 359 tra genes for components of conjugative type IV secretion systems were identified at 79 sites in the genome. Associated with these are >200 genes for signaling and host-cell interaction proteins, such as histidine kinases, ankyrin-repeat proteins, and tetratrico peptide-repeat proteins. Additionally, the O. tsutsugamushi genome contains >400 transposases, 60 phage integrases, and 70 reverse transcriptases. Deletions and rearrangements have yielded unique gene combinations as well as frequent pseudogenization in the tra clusters. A comparative analysis of the tra clusters within the genome and across strains indicates sequence homogenization by gene conversion, whereas complexity, diversity, and pseudogenization are acquired by duplications, deletions, and transposon integrations into the amplified segments. The results suggest intragenomic duplications or multiple integrations of a massively proliferating conjugative transfer system. Diversifying selection on host-cell interaction genes along with repeated population bottlenecks may drive rare genome variants to fixation, thereby short-circuiting selection for low complexity in bacterial genomes. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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