التفاصيل البيبلوغرافية
| العنوان: |
Hyposmotic challenge inhibits inward rectifying K+ channels in cerebral arterial smooth muscle cells. |
| المؤلفون: |
Bin-Nan Wu, Luykenaar, Kevin D., Brayden, Joseph E., Giles, Wayne R., Corteling, Randolph L., Wiehler, William B., Welsh, Donald G. |
| المصدر: |
American Journal of Physiology: Heart & Circulatory Physiology; Feb2007, Vol. 292 Issue 2, pH1085-H1094, 10p, 10 Graphs |
| مصطلحات موضوعية: |
CEREBRAL arteries, SMOOTH muscle, MUSCLE cells, ELECTROPHYSIOLOGY, RATS |
| مستخلص: |
This study sought to define whether inward rectifying K+ (KIR) channels were modulated by vasoactive stimuli known to depolarize and constrict intact cerebral arteries. Using pressure myography and patch-clamp electrophysiology, initial experiments revealed a Ba2+-sensitive KIR current in cerebral arterial smooth muscle cells that was active over a physiological range of membrane potentials and whose inhibition led to arterial depolarization and constriction. Real-time PCR, Western blot, and immunohistochemical analyses established the expression of both KIR2.1 and KIR2.2 in cerebral arterial smooth muscle cells. Vasoconstrictor agonists known to depolarize and constrict rat cerebral arteries, including uridine triphosphate, U46619, and 5-HT, had no discernable effect on whole cell KIR activity. Control experiments confirmed that vasoconstrictor agonists could inhibit the voltage-dependent delayed rectifier K+ (KDR) current. In contrast to these observations, a hyposmotic challenge that activates mechanosensitive ion channels elicited a rapid and sustained inhibition of the KIR but not the KDR current. The hyposmotic-induced inhibition of KIR was 1) mimicked by phorbol-12-myristate-13-acetate, a PKC agonist; and 2) inhibited by calphostin C, a PKC inhibitor. These findings suggest that, by modulating PKC, mechanical stimuli can regulate KIR activity and consequently the electrical and mechanical state of intact cerebral arteries. We propose that the mechanoregulation of KIR channels plays a role in the development of myogenic tone. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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