التفاصيل البيبلوغرافية
| العنوان: |
Müller cell expression of glutamate cycle related proteins and anti-apoptotic proteins in early human retinal development. |
| المؤلفون: |
Georges, P., Cornish, E. E., Provis, J. M., Madigan, M. C. |
| المصدر: |
British Journal of Ophthalmology; Feb2006, Vol. 90 Issue 2, p223-238, 6p, 7 Diagrams, 1 Chart |
| مصطلحات موضوعية: |
PROTEINS, RETINA, POSTERIOR segment (Eye), GLUTAMINE synthetase, LIGASES, AMINO acids |
| مستخلص: |
Aims: The distribution of glutamate cycle related proteins (glutamine synthetase (GS) and GLAST) and anti-apoptotic proteins (Bcl-2 and Bcl-X) was investigated in Müller cells during early human retinal development, relative to the onset of expression of synaptophysin, a presynaptic vesicle protein. Methods: Using frozen sections of human fetal eyes (13–22 weeks gestation) (n = 10), Bcl-2, Bcl-X, GS, GLAST, and synaptophysin immunoreactivities (IR) were imaged using fluorescence microscopy and plowed as a function of eccentricity from the incipient fovea. Frozen sections of adult human retina (n = 4) were immunolabelled with antibodies to Bcl-2 and Bcl-X. Results: Müller cell immunoreactivity for GS, GLAST, and Bcl-2 was initially detected in the incipient fovea, and then at more peripheral locations with increasing age. Synaptophysin-IR appeared earlier than all other target proteins. Within the synaptophysin-IR region, mature (differentiated) Müller cells expressed both Bcl-2 and Bcl-X-IR from 13 weeks gestation, ahead of GS-IR and GLAST-IR that were first seen at 14 weeks gestation. Additionally, from as early as 13 weeks gestation, ganglion cells and immature neuronal progenitor cells across the entire retina expressed Bcl-2-1R and Bcl-X-IR, respectively. In adult retina, ganglion cells and some bipolar cells expressed Bcl-X but not Bcl-2. Conclusion: Müller cells express Bcl-2 and Bcl-X after synaptogenesis has commenced, but before the onset of GS and GLAST expression, suggesting a protective role for these proteins in Müller cells during the onset of glutamatergic transmission in early human retinal development. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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