التفاصيل البيبلوغرافية
| العنوان: |
Evaluation of Yellow Fever Virus Infection in Aedes aegypti Mosquitoes from Pakistan with Distinct Knockdown Resistance Genotypes. |
| المؤلفون: |
dos Santos, Carlucio Rocha, Santos, Caleb Guedes Miranda dos, Couto-Lima, Dinair, Souza, Bárbara Silva, Rahman, Rafi Ur, Dornelas Ribeiro, Marcos, Lima, José Bento Pereira, Martins, Ademir Jesus |
| المصدر: |
Insects (2075-4450); Jan2025, Vol. 16 Issue 1, p33, 17p |
| مصطلحات موضوعية: |
AEDES aegypti, YELLOW fever, VIRAL genetics, GENETIC variation, ENDEMIC diseases |
| مستخلص: |
Simple Summary: Background: Yellow fever (YF) is a serious disease found in Africa and Latin America that is potentially transmitted by Aedes aegypti in urban regions. Surprisingly, YF did not occur in Asia, despite the presence of these mosquitoes. This may be related to environmental factors, genetic aspects of the virus strains and mosquito populations. Moreover, as insecticide resistance mutations, such as kdr, influence mosquito physiology, such mutations might affect its infectivity by the yellow fever virus (YFV). Therefore, we examined whether mosquitoes from Pakistan with distinct kdr genotypes could be infected and replicate the YF virus. Methods: Female Aedes aegypti from Pakistan were exposed to two YFV strains in the laboratory. A laboratory mosquito strain was used for comparison. After two weeks, the mosquitoes were tested to determine if the virus had spread through their bodies. Genetic analysis was performed to identify kdr mutations related to insecticide resistance. Results: Pakistani mosquitoes were susceptible to YFV, with infection rates similar to those of laboratory strains. Two kdr mutations were detected; however, these mutations did not affect the ability of mosquitoes to become infected with YFV. Conclusions: Ae. aegypti mosquitoes from Pakistan can become infected with YFV even if they possess mutations associated with insecticide resistance. Background: Yellow fever (YF) is an acute hemorrhagic disease endemic to Africa and Latin America; however, no cases have been reported in Asian regions with high Aedes aegypti infestation. Factors such as environmental conditions and genetic variations in the yellow fever virus (YFV) strains and mosquito populations may explain this absence. Mosquito populations have undergone strong selective pressure owing to the excessive use of insecticides. This pressure has led to the spread of alterations, such as knockdown-resistant mutations (kdr), which, while conferring resistance to pyrethroids, also induce various physiological side effects in the insect. Therefore, it is important to investigate whether the presence of kdr mutations influences the infectivity of YFV mosquitoes. This study evaluated the susceptibility of Ae. aegypti from Pakistan with distinct kdr genotypes to different YFV strains under laboratory conditions. Methods: Ae. aegypti from a Pakistani colony were exposed to YFV strains (PR4408/2008 and ES504/2017) along with the Rockefeller strain. After 14 days, RNA and DNA were extracted for viral RNA detection (qPCR) and kdr genotyping (TaqMan qPCR and HRM for T1520I and F1534C SNPs). Results: Pakistani Ae. aegypti were orally susceptible to YFV, with infection rates of 83.7% (PR4408/2008) and 61.3% (ES504), respectively, similar to Rockefeller. Two kdr genotypes (II + CC and TI + FC) were identified, with no significant differences in viral infection or dissemination rates. Conclusions: The Ae. aegypti population from Asia is capable of YFV infection and dissemination, regardless of kdr genotype. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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