التفاصيل البيبلوغرافية
| العنوان: |
TLC Identification and Extraction Process of Rubiasin-1-methyl Ether from Yao Medicine Chuanlianzhu. |
| المؤلفون: |
Jingrong LUA, Jiangcun WE, Xiumei MA, Bing QING, Meiyan QIU, Wen ZHONG |
| المصدر: |
Medicinal Plant; Jun2024, Vol. 15 Issue 3, p35-38, 4p |
| مصطلحات موضوعية: |
ULTRAVIOLET lamps, GRADIENT elution (Chromatography), SILICA gel, PHOSPHORIC acid, CHROMATOGRAPHIC analysis |
| مستخلص: |
[Objectives] To establish a thin-layer chromatography (TLC) method for the determination of rubiadin-1-methyl ether in Yao Medicine Chuanlianzhu (Damnacanthus giganteus). [Methods] A silica gel G thin-layer plate was adopted for TLC. Petroleum ether (60 -90 °C') -chloroform-methanol-water (7:15:3:1) was used as the developing solvent and inspected under ultraviolet lamp (365 nm). The content was determined by Inertsil ODS-3 Cla column (4.60 mm x250 mm, 5 (jim), mobile phase; acetonitrile-0. 2% phosphoric acid gradient elution, detection wavelength 277 nm, flow rate 1.0 mL/min, column temperature 30 °C, injection volume 10 |jlL. [Results] The spots of 10 Chuanlianzhu samples from different origins showed the same color at the same position as the control, and the spots were clear and specific. The injection volume of rubiadin-1 -methyl ether showed a good linear relationship in the range of 2.90 -145 p,g (R =0.999 6). The average recovery rate of rubiadin-1-methyl ether in the low, medium and high dose groups of Yao Medicine Chuanlianzhu was 98.72%, and RSD =1.78%. [Conclusions] This method can effectively identify Yao Medicine Chuanlianzhu medicinal materials and accurately determine the content of rubiadin-1 -methyl ether in the medicinal materials. It provides a scientific basis for the development and utilization of Yao Medicine Chuanlianzhu medicinal resources. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |