التفاصيل البيبلوغرافية
| العنوان: |
Detection of Mycobacterium chimaera in medical device water samples by customised real time PCR using a InGenius platform. |
| المؤلفون: |
Bisognin, Francesco, Borghi, Michele, Faccio, Matteo, Ferraro, Vincenzo, Sorella, Federica, Crovara Pesce, Cristina Maria, Primavera, Alessandra, Dal Monte, Paola |
| المصدر: |
Scientific Reports; 12/28/2024, Vol. 14 Issue 1, p1-6, 6p |
| مصطلحات موضوعية: |
MYCOBACTERIUM avium, POLYMERASE chain reaction, WATER sampling, WATER heaters, MYCOBACTERIUM |
| مستخلص: |
Mycobacterium chimaera, belonging to the Mycobacterium avium complex, is an opportunistic environmental mycobacterium which has been isolated from medical device water samples such as Heater Cooler Units (HCU). Laboratories currently use culture-based diagnostic methods to detect M. chimaera, but these take a long time to obtain results. The aim of this study is to test and define the Limit of Detection (LoD) of a Real-Time Polymerase Chain Reaction test (RT-PCR) specific for M. chimaera, using a sample-to-result InGenius platform, performed on medical device water samples collected at the referral centre for the detection of mycobacteria from environmental specimens of Bologna, Italy. A total of 285 water samples were included in this study. The sensitivity and specificity of RT-PCR compared to culture were 60.5% and 98.8% respectively, with an overall agreement of 82.1% and a positive predictive value of 97.4%. The LoD calculated was approximately 2900 CFU/ml. In conclusion, this study confirmed that detection of M. chimaera with RT-PCR could support culture-based methods in reducing the time necessary to identify highly colonised HCUs, with high positive predictive values. Therefore, we suggest performing this customised RT-PCR on concentrated decontaminated water samples, shutting down and thoroughly disinfecting positive HCUs, to reduce the risk of patient infection. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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