التفاصيل البيبلوغرافية
| العنوان: |
砷脂促进秀丽隐杆线虫衰老的毒性 效应及其分子机制. (Chinese) |
| Alternate Title: |
Toxic Effect and Molecular Mechanism of Arsenolipids on Promoting Aging in Caenorhabditis elegans. (English) |
| المؤلفون: |
尹 欢, 宋兵兵, 黎梓玉, 汪 卓, 赵巧丽, 李 瑞, 陈建平, 钟赛意 |
| المصدر: |
Shipin Kexue/ Food Science; 2024, Vol. 45 Issue 23, p131-139, 9p |
| مصطلحات موضوعية: |
POISONS, TRANSCRIPTION factors, ANIMAL clutches, MOLECULAR weights, PROTEIN expression |
| Abstract (English): |
In this study, we explored the effect of two arsenolipids with different molecular masses, AsHC 332 and AsHC 346, which are commonly found in seafood, on aging and lifespan-related indicators in the model organism Caenorhabditis elegans. The possible mechanism by which the arsenolipids promote aging was analyzed by measuring the expression of DAF-16 protein in the nematode, the longevity of aging mutants and stress-related genes. The results showed that AsHCs significantly reduced the body length, body width, brood size and head thrash frequency of C. elegans in a dose-dependent manner (P < 0.05). Moreover, the resistance to heat stress (P < 0.000 1) and ultraviolet stress (P < 0.05) was significantly reduced by AsHCs. The lifespan of C. elegans was significantly shortened (P < 0.01). The accumulation of lipofuscin was increased (P < 0.001). The relative expression of the daf-16, sod-3, ctl-1, gst-4 and hsp-16.2 genes was up-regulated, while the nuclear localization of DAF-16 was not significantly promoted, and there was no effect on the lifespan of the daf-16(mu86) mutant. AsHC 332 and AsHC 346 had the same toxic effects on nematode aging, which may shorten the lifespan of C. elegans by regulating the transcription factor DAF-16. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
以秀丽隐杆线虫 (Caenorhabditis elegans) 为模型, 研究AsHC 332和AsHC 346等常见于海产品中两种不同 分子质量砷脂对线虫衰老和寿命相关指标的影响, 并通过测定对线虫DAF-16蛋白表达情况、衰老突变株寿命和应 激相关基因的影响分析其促衰老的可能机制。结果表明, 砷脂以浓度依赖性的方式显著降低了线虫体长、体宽、后 代数目和头部摆动频率 (P＜0.05); 降低了线虫对热应激 (P＜0.000 1) 和紫外应激抗逆性 (P＜0.05); 显著缩 短了线虫寿命 (P＜0.01); 增加了线虫脂褐素积累 (P＜0.001); 上调了daf-16、sod-3、ctl-1、gst-4和hsp-16.2基 因相对表达, 对线虫DAF-16蛋白核定位促进作用不显著, 对daf-16(mu86)突变株寿命无影响。研究表明AsHC 332和 AsHC 346对线虫衰老的毒性效应相同, 可能依赖于DAF-16转录因子的调节, 从而缩短线虫寿命。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |