التفاصيل البيبلوغرافية
| العنوان: |
Effects of Glutamine or Glucose Deprivation on Inflammation and Tight Junction Disruption in Yak Rumen Epithelial Cells. |
| المؤلفون: |
Yue, Ziqi, Wang, Junmei, Hu, Rui, Peng, Quanhui, Guo, Hongrui, Zou, Huawei, Xiao, Jianxin, Jiang, Yahui, Wang, Zhisheng |
| المصدر: |
Animals (2076-2615); Nov2024, Vol. 14 Issue 22, p3232, 19p |
| مصطلحات موضوعية: |
TIGHT junctions, TELOMERASE reverse transcriptase, SV40 (Virus), CELL morphology, MITOGEN-activated protein kinases, MITOGENS |
| مستخلص: |
Simple Summary: Nutrient deficiency in yak has become a common phenomenon because of pasture deficiency in the cold season in the plateau regions. In ruminants, the rumen plays an important function in the digestion and absorption of nutrients. The previous study of our research group reported that forage deficiency significantly decreased the concentration of glutamine (Gln), thus leading to the inflammation and barrier function damage of yak ruminal epithelium tissue in the cold season, which inhibited yak growth. However, the specific molecular mechanisms are still unclear. Thus, we explored the mechanism of the effects and the differences between the Gln and glucose (an important energy source, GLU) deprivation on epithelial cell function. The results showed that Gln deprivation (Gln-D) beyond 12 h disrupted YREC cellular tight junctions by inducing oxidative stress and glucose deprivation (GLU-D) beyond 12 h disrupted yak rumen epithelial cell (YRECs) cellular tight junctions by inducing apoptosis and oxidative stress. Meanwhile, Gln-D or GLU-D significantly improved the p38 mitogen-activated protein kinase and JNK protein expression levels. Consequently, the harmful effect of Gln-D or GLU-D might be associated with the activation of both the p38 MAPK and JNK signaling pathways in YRECs. Also, GLU-D induced injury more serious to YRECs than Gln-D. Furthermore, GLU-D reduced the levels of tight junction gene expression in YRECs compared with Gln-D. Yak is a special free-ranging cattle breed in the plateau areas of Qinghai and Tibet. Pasture withering in cold-season pastures results in energy deficiency in yaks, which undermines the rumen epithelial barrier. However, the leading factor causing rumen epithelial injury remains unknown. Glutamine (Gln), a conditionally essential amino acid, is insufficient under pathological conditions. Glucose (GLU) is an important energy source. Thus, we explored the effects of Gln or GLU deprivation on the barrier function of yak rumen epithelial cells and investigated the underlying mechanisms, as well as the differences in rumen epithelial barrier function between Gln deprivation (Gln-D) and GLU deprivation (GLU-D). In previous work, we constructed the yak rumen epithelial cells (YRECs) line by transferring the human telomerase reverse transcriptase gene (hTERT) and simian virus 40 large T antigen (SV40T) into primary YRECs. The YRECs were exposed to normal, Gln-D, GLU-D, and serum replacement (SR) media for 6, 12, and 24 h. Our data displayed that cell viability and tight junction protein expression in the SR group were not significantly changed compared to the normal group. Whereas, compared with the SR group, Gln-D treated for more than 12 h reduced cell viability and proliferation, and GLU-D treated for more than 12 h damaged the cell morphology and reduced cell viability and proliferation. The cell proliferation and cell viability were decreased more in GLU-D than in Gln-D. In addition, Gln-D treated for more than 12 h disrupted YREC cellular partially tight junctions by inducing oxidative stress and inflammation, and GLU-D treated for more than 12 h disrupted YREC cellular tight junctions by inducing apoptosis, oxidative stress, and inflammation. Compared with Gln-D, GLU-D more significantly induced cell injury and reduced tight junction protein levels. Our results provided evidence that GLU-D induced damage through the p38 mitogen-activated protein kinase (p38 MAPK)/c-junN-terminal kinase (JNK) signaling pathway, which was more serious than Gln-D treated for more than 12 h. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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