التفاصيل البيبلوغرافية
| العنوان: |
Ultra-inert lanthanide chelates as mass tags for multiplexed bioanalysis. |
| المؤلفون: |
David, Tomáš, Šedinová, Miroslava, Myšková, Aneta, Kuneš, Jaroslav, Maletínská, Lenka, Pohl, Radek, Dračínský, Martin, Mertlíková-Kaiserová, Helena, Čížek, Karel, Klepetářová, Blanka, Litecká, Miroslava, Kaňa, Antonín, Sýkora, David, Jaroš, Adam, Straka, Michal, Polasek, Miloslav |
| المصدر: |
Nature Communications; 11/14/2024, Vol. 15 Issue 1, p1-12, 12p |
| مصطلحات موضوعية: |
COORDINATION compounds, CELL-penetrating peptides, RARE earth metals, CONTRAST media, CHELATES |
| مستخلص: |
Coordination compounds of lanthanides are indispensable in biomedical applications as MRI contrast agents and radiotherapeutics. However, since the introduction of the chelator DOTA four decades ago, there has been only limited progress on improving their thermodynamic stability and kinetic inertness, which are essential for safe in vivo use. Here, we present ClickZip, an innovative synthetic strategy employing a coordination-templated formation of a 1,5-triazole bridge that improves kinetic inertness up to a million-fold relative to DOTA, expanding utility of lanthanide chelates beyond traditional uses. Acting as unique mass tags, the ClickZip chelates can be released from (biological) samples by acidic hydrolysis, chromatographically distinguished from interfering lanthanide species, and sensitively detected by mass spectrometry. Lanthanides enclosed in ClickZip chelates are chemically almost indistinguishable, providing a more versatile alternative to chemically identical isotopic labels for multiplexed analysis. The bioanalytical potential is demonstrated on tagged cell-penetrating peptides in vitro, and anti-obesity prolactin-releasing peptides in vivo. Lanthanide coordination compounds are indispensable in biomedical applications yet there has been limited progress on improving their thermodynamic stability and kinetic inertness. Here, the authors report the coordination-templated formation of a 1,5-triazole bridge that improves kinetic inertness up to a million-fold relative to DOTA. [ABSTRACT FROM AUTHOR] |
|
Copyright of Nature Communications is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder