التفاصيل البيبلوغرافية
| العنوان: |
アルカリ性塩溶液による効率的な ルイボス由来UVC 保護物質の回収. (Japanese) |
| Alternate Title: |
Efficient extraction of anti-UVC substances from Rooibos by alkaline salt solutions. (English) |
| المؤلفون: |
井澤 真希, 大高 祐聖, 坂上 宏, 魚田 慎, 須永 克佳, 鈴木 龍一郎, 芝 規良, 高橋 伸年, 﨑山 浩司, 河野 哲, 田 いづみ, 藤原 周, 中嶌 裕, 大友 克之, 宮田 淳, Indermun, Suvarna, Yengopal, Veerasamy, Bawa, Umesh, Jeppie, Ghaleeb, 鬼頭 慎司 |
| المصدر: |
New Food Industry; 2024, Vol. 66 Issue 12, p751-762, 12p |
| مصطلحات موضوعية: |
ALKALINE solutions, CYTOTOXINS, GREEN tea, HOT water, FLAVONOIDS |
| Abstract (English): |
While ultraviolet C (UVC) has strong bactericidal and virucidal activity, the excessive exposure to UVC may increase harmful effects on human body, therefore, the search for effective UVC protective substances is crucial. Rooibos, a type of water-soluble herb, contains the antioxidant aspalathin of inherent flavonoid, and depending on the extraction method, it may have anti-UVC effects. In this study, we compared the anti-UCV activities of PET bottles, hot-water extract and alkaline extract of Rooibos, using normal human dermal fibroblasts (HDFa) and human melanoma cells (COLO679). Cells in culture medium without or with rooibos extract were exposed to UVC and cell viability was measured with MTT assay. Anti-UVC activity was determined by selective index (SI), that is the ratio of CC50 (50% cytotoxic concentration against unirradiated cells) to EC50 (concentration that restored UVC-induced loss of viability by 50%). PET bottle of Rooibos showed more stable, and broader range of anti-UVC activity due to lower cytotoxicity, as compared with that of green tea and black tea. Both fermented Rooibos and non-fermented Rooibos exhibited anti-UCV activity in short-term culture, however, in long-term culture, their anti-UVC activity were no longer detected due to cytotoxicity. The alkaline solution (pH 12) efficiently extracts lignin with high anti-UVC activity, but breaks ester bonds to decompose lignin. Therefore, when a weakly alkaline solution (pH 9.0) was used, about twice as much the anti-UVC active substance could be recovered as compared with using hot water extract. As a future project, the optimization of conditions (such as temperature, pH and time) for alkali extraction should be considered. [ABSTRACT FROM AUTHOR] |
| Abstract (Japanese): |
紫外線C（UVC）は, 強力な殺菌力と殺ウイルス力を持つ反面, UVC を過度に吸収した場合, 人体への 有害な影響が増大する可能性があり, 効果的な UVC 保護物質の探索が必要不可欠である。水溶性ハーブの 一種であるRooibos は, 抗酸化の強いアスパラチンなどのフラボノイド類を含み, 抗UVC 効果が期待できる。 本研究では, Rooibos の①ペットボトル, ②熱水抽出液, ③アルカリ抽出液の抗UVC 活性を, 正常ヒト 皮膚線維芽細胞（HDF）とヒト黒色腫細胞（COLO679）を用いて比較検討した。細胞を, ルイボス抽出物 を含む培養液に置換し, UVC 照射後, 細胞生存率をMTT 法を用いて測定した。抗UVC 活性(SI 値) は, CC50 値（非照射細胞で測定した50％細胞毒性濃度）とEC50 値（UVC 照射により減少した生細胞数を半分 回復させる濃度）の比（SI=CC50/EC50）により定量した。ペットボトルでは, Rooibos は, 紅茶や緑茶より も, 抗UVC 活性を与える濃度範囲が広く, かつ細胞毒性が低かった。熱水抽出物は発酵Rooibos, 非発酵 Rooibos ともに, 短時間処理では抗UCV 活性を示すが, 長時間処理では細胞傷害作用が発現し, 抗UVC 活性が検出できなくなった。アルカリ性溶液(pH12) は, 抗UVC 活性の高いリグニンを抽出するとともに, エステル結合を切断してリグニンを分解・消失させてしまう。そこで, 弱アルカリ性溶液(pH 9.0) による 抽出を試みたところ, 細胞傷害性物資の除去および熱水抽出液の約２倍の抗UVC 活性物質を回収できるこ とが推定された。今後の検討課題として, アルカリ抽出の条件（温度, pH, 時間）の至適化を考えている. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |