التفاصيل البيبلوغرافية
| العنوان: |
The power of 810 nm near-infrared photobiomodulation therapy for human asthenozoospermia. |
| المؤلفون: |
Stigliani, Sara, Ravera, Silvia, Maccarini, Elena, Rizzo, Camilla, Massarotti, Claudia, Anserini, Paola, Bozzo, Matteo, Amaroli, Andrea, Scaruffi, Paola |
| المصدر: |
Scientific Reports; 11/5/2024, Vol. 14 Issue 1, p1-15, 15p |
| مصطلحات موضوعية: |
PHOTOBIOMODULATION therapy, SPERM motility, AEROBIC metabolism, SEMICONDUCTOR lasers, OXIDATIVE phosphorylation |
| مستخلص: |
Sperm motility is a crucial factor in male fertility. Photobiomodulation (PBM) has been reported to increase sperm motility, but a consistent approach suitable for identifying standardizable protocols is lacking. We collected asthenozoospermic (n = 70) and normozoospermic (n = 20) semen. The asthenozoospermic samples were irradiated with an 810 nm diode laser, in continuous wave mode, at 0.25 W, 0.5 W, 1 W and 2 W for 60 s on a circular area of 1 cm2 through a novel handpiece with an innovative flat-top profile. Sperm motility was assessed immediately, after 30 and 60 min. A sample size calculator, unpaired t-test and one-way ANOVA with post-hoc Tukey HSD tests were used for statistics. One and 2 W were the most effective outputs in increasing progressive motility compared to control (p < 0.001). The maximum effect was immediately after 1 W-PBM (p < 0.001) and decreased after 60 min (p < 0.001). Time physiologically decreased vitality (p < 0.001), but less in the 1 W-PBM samples (p < 0.05). 1 W-PBM did not affect chromatin condensation. Asthenozoospermic samples displayed an impairment of 80% in oxygen consumption and ATP production and a slight inefficiency of oxidative phosphorylation compared to normozoospermic samples (p < 0.001). 1 W-PBM partially restored the functionality of aerobic metabolism (p < 0.001) by recovery of oxidative phosphorylation efficiency. PBM did not affect lactate dehydrogenase (glycolysis pathway). No irradiated samples increased accumulated malondialdehyde, a marker of lipidic peroxidation. In conclusion, PBM improves progressive motility in asthenozoospermia through increased mitochondrial energetic metabolism without harmful oxidative stress. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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