التفاصيل البيبلوغرافية
| العنوان: |
Structural diversity and clustering of bacterial flagellar outer domains. |
| المؤلفون: |
Fields, Jessie Lynda, Zhang, Hua, Bellis, Nathan F., Petersen, Holly A., Halder, Sajal K., Rich-New, Shane T., Krupovic, Mart, Wu, Hui, Wang, Fengbin |
| المصدر: |
Nature Communications; 11/3/2024, Vol. 15 Issue 1, p1-12, 12p |
| مصطلحات موضوعية: |
STENOTROPHOMONAS maltophilia, ATOMIC structure, BACTERIAL diversity, FLAGELLIN, FLAGELLA (Microbiology) |
| مستخلص: |
Supercoiled flagellar filaments function as mechanical propellers within the bacterial flagellum complex, playing a crucial role in motility. Flagellin, the building block of the filament, features a conserved inner D0/D1 core domain across different bacterial species. In contrast, approximately half of the flagellins possess additional, highly divergent outer domain(s), suggesting varied functional potential. In this study, we report atomic structures of flagellar filaments from three distinct bacterial species: Cupriavidus gilardii, Stenotrophomonas maltophilia, and Geovibrio thiophilus. Our findings reveal that the flagella from the facultative anaerobic G. thiophilus possesses a significantly more negatively charged surface, potentially enabling adhesion to positively charged minerals. Furthermore, we analyze all AlphaFold predicted structures for annotated bacterial flagellins, categorizing the flagellin outer domains into 682 structural clusters. This classification provides insights into the prevalence and experimental verification of these outer domains. Remarkably, two of the flagellar structures reported herein belong to a distinct cluster, indicating additional opportunities on the study of the functional diversity of flagellar outer domains. Our findings underscore the complexity of bacterial flagellins and open up possibilities for future studies into their varied roles beyond motility. Here the authors use cryo-EM to determine the structures of three bacterial flagellar filaments, revealing distinct outer domains. Upon further analysis of all AlphaFold predicted flagellar outer domains, they show that the outer domains of flagella are highly diverse. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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