التفاصيل البيبلوغرافية
| العنوان: |
一株产纤维素酶酵母菌的筛选、鉴定及产酶条件优化. (Chinese) |
| Alternate Title: |
Screening, identification and enzyme production conditions optimization of a cellulase-producing yeast. (English) |
| المؤلفون: |
冉光耀, 唐佳代, 赵益梅, 孟卓妮, 龙亚飞, 郭敏, 郭举 |
| المصدر: |
China Brewing; 2024, Vol. 43 Issue 9, p72-78, 7p |
| مصطلحات موضوعية: |
ROSE bengal, MOLECULAR biology, CONGO red (Staining dye), FILTER paper, CELLULASE |
| Abstract (English): |
In order to screen high cellulose-producing yeast, cellulose-producing yeasts were screened from the fermented grains of sauce-flavor (Jiangxiangxing) Baijiu by Rose Bengal Agar and Congo Red Medium, and the screened strains were identified by morphological observation, physiological and biochemical tests, and molecular biology technique. The tolerance was investigated and the enzyme production conditions were optimized. The results showed that a cellulase-producing strain JXJP-Y19 was screened, and the strain JXJP-Y19 was identified as Yarrowia lipolytica. The temperature, ethanol, and pH tolerance of the strain JXJP-Y19 were 39 °C, 8%(V/V), and 3, respectively, showing good tolerance to environmental stress. The optimal enzyme production conditions were as follows: bran as carbon source, cottonseed cake as nitrogen source, inoculum 1%, initial pH 6 and fermentation temperature 30 °C. Under these optimal conditions, the activities of filter paper enzyme, exoglucanase and ẞ-glucosidase produced by the strain JXJP-Y19 were (0.913±0.025) U/ml, (1.272±0.025) U/ml and (0.937±0.030) U/ml, respectively, which were 2.15 times, 1.81 times, and 1.94 times higher than before optimization. The strain JXJP-Y19 showed good cellulase activity and could be used as a potential strain for cellulose bio-degradation utilization. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
为了筛选高产纤维素酶酵母菌, 该研究利用孟加拉红培养基和刚果红培养基, 从酱香型白酒酒醅中筛选产纤维素酶的酵母 菌, 通过形态学观察、生理生化试验及分子生物学技术对筛选菌株进行鉴定, 并考察其耐受性并优化产酶条件。结果表明, 筛选出一株 产纤维素酶菌株 JXJP-Y19, 经鉴定后确定该菌株为解脂耶氏酵母菌 (Yarrowia lipolytica)。菌株 JXJP-Y19 的温度、乙醇、pH 耐受性分别 为 39℃、8%(V/V)、3, 整体表现出良好的环境胁迫耐受性能。最佳产酶条件为以麸皮作碳源, 棉籽饼作氮源, 接种量1%, 初始pH值6, 发酵温度30℃。在此优化条件下, 菌株 JXJP-Y19 所产滤纸酶活力、外切葡聚糖酶和 β-葡萄糖苷酶酶活分别为 (0.913±0.025) U/mL、 (1.272±0.025) U/mL、(0.937±0.030) U/mL, 是优化前的2.15 倍、1.81 倍和 1.94 倍。菌株 JXJP-Y19 展现出良好的纤维素酶活力, 可作为潜 在菌株应用于纤维素生物降解利用。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |