التفاصيل البيبلوغرافية
| العنوان: |
基于脑肠轴探讨益气活血化浊解毒方对脑缺血再灌注损伤大鼠的影响. (Chinese) |
| Alternate Title: |
Effect of Yiqi Huoxue Huazhuo Jiedu Prescription on treatment of cerebral ischemia reperfusion injury rats based on brain-gut axis. (English) |
| المؤلفون: |
孙亚萍, 石瑞, 孙玲玲, 谢占维, 崔怡娴, 田军彪 |
| المصدر: |
Chinese Journal of Immunology; Aug2024, Vol. 40 Issue 8, p1709-1717, 9p |
| مصطلحات موضوعية: |
REPERFUSION injury, BACTERIAL metabolites, SIZE of brain, GUT microbiome, TIGHT junctions, MYOCARDIAL reperfusion, INTESTINAL injuries |
| Abstract (English): |
Objective: To investigate effect of Yiqi Huoxue Huazhuo Jiedu Prescription （YHHJP） on inflammatory factors of brain tissues, tight junction between brain and colon tissues, intestinal flora and bacterial metabolites in cerebral ischemia reperfusion injury （CIRI） rats based on brain-gut axis. Methods: Fifty male SD rats of SPF grade were randomly divided into sham-operation group （Sham）, model group （MCAO）, low, medium, high doses YHHJP groups （TCM-L/TCM-M/TCM-H）. Middle cerebral artery occlusion model was established according to Zea Longa methods. Neurological function defects were detected 3 days after administration. TTC staining was used to calculate infarct size of brain tissue. Pathological changes of brain tissue were observed by Nissl staining, and pathological changes of brain and colon tissues were observed by HE staining. IL-1β, IL-6, TNF-α in brain tissue and LPS content in serum were detected by ELISA, and D-LA content in serum was detected by biochemical method. Gene expressions of ZO-1 and Claudin-5 in brain tissue and gene expressions of ZO-1, Claudin-1 in colon tissue were studied by Real-time fluorescent quantitative PCR. Intestinal flora were detected by 16S rDNA high-throughput sequencing. Results: Compared with Sham group, pathological damage of brain and colon tissue were serious in MCAO group, intestinal flora structure was significantly different, neural function impairment was aggravated, infarct size was increased, IL-1β, IL-6, TNF-α contents in brain tissue, and LPS, D-LA contents in serum were increased, gene expressions of ZO-1 and Claudin-5 in brain tissue and gene expressions of ZO-1 and Claudin-1 in colon tissue were decreased significantly （P<0.05）. Compared with MCAO group, pathological damage of brain and colon tissue of rats were relieved in TCM-L, TCM-M, TCM-H groups, disturbance of intestinal microflora microecology was improved, neurological function impairment and infarct size were markedly decreased, IL-1β, IL-6, TNF-α contents in brain tissue were decreased, gene expressions of ZO-1 and Claudin-1 in colon tissue were increased significantly （P<0.05）; LPS and D-LA contents in serum were decreased in YHHJP medium and high doses groups, while gene expressions of ZO-1 and Claudin-5 in brain tissue were increased significantly （P< 0.05）. Conclusion: YHHJP has a good effect on improving CIRI, whose mechanism may be related to regulating diversity of intestinal flora, reducing release of intestinal bacterial metabolites LPS and D-LA, increasing gene expressions of tight junction proteins ZO-1, Claudin-5 and Claudin-1, and down-regulating secretion of proinflammatory cytokine. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
目的: 基于脑肠轴探讨益气活血化浊解毒方（YHHJP）对脑缺血再灌注损伤（CIRI）大鼠脑组织炎症因子、脑 和结肠组织紧密连接、肠道菌群及其代谢产物的影响。方法: 50只SPF级雄性SD大鼠随机分为假手术组（Sham）、模型组 （MCAO）、YHHJP低、中、高剂量组（TCM-L/TCM-M/TCM-H）。采用Zea Longa线栓法制备大鼠大脑中动脉闭塞再灌注模型, 治 疗3 d后观察神经功能缺损评分, TTC染色计算脑梗死面积, 尼氏染色观察脑组织病理形态改变, HE染色观察脑和结肠组织病 理形态改变, ELISA检测脑组织IL-1β、IL-6、TNF-α和血清LPS含量, 生物化学法检测血清D-乳酸（D-LA）含量, 实时荧光定量 PCR检测脑组织ZO-1、Claudin-5和结肠组织ZO-1、Claudin-1基因表达, 16S rDNA高通量测序检测肠道菌群。结果: 与假手术 组相比, 模型组大鼠脑和结肠组织病理损伤严重, 肠道菌群微生态结构存在明显差异, 神经功能损伤加重, 脑梗死面积增加, 脑 组织IL-1β、IL-6、TNF-α 和血清LPS、D-LA 含量升高, 脑组织ZO-1、Claudin-5 及结肠组织ZO-1、Claudin-1 基因表达降低（P< 0.05）。与模型组相比, YHHJP低、中、高剂量组大鼠脑组织和结肠组织病理损伤减轻, 肠道菌群微生态紊乱得到改善, 神经功 能损伤减轻, 脑梗死面积明显缩小, 脑组织IL-1β、IL-6、TNF-α含量显著降低, 结肠组织ZO-1、Claudin-1基因表达显著升高（P< 0.05）;YHHJP中、高剂量组大鼠血清LPS、D-LA含量明显降低, 脑组织ZO-1、Claudin-5基因表达显著升高（P<0.05）。结论: YHHJP可改善CIRI, 其机制可能与调节肠道菌群多样性, 抑制肠道细菌代谢产物LPS、D-LA释放, 增加紧密连接蛋白ZO-1、 Claudin-5、Claudin-1基因表达, 下调炎症因子分泌有关。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |