التفاصيل البيبلوغرافية
| العنوان: |
Structural Assessment of Chlamydia trachomatis Major Outer Membrane Protein (MOMP)-Derived Vaccine Antigens and Immunological Profiling in Mice with Different Genetic Backgrounds. |
| المؤلفون: |
Roe, Shea K., Zhu, Tianmou, Slepenkin, Anatoli, Berges, Aym, Fairman, Jeff, de la Maza, Luis M., Massari, Paola |
| المصدر: |
Vaccines; Jul2024, Vol. 12 Issue 7, p789, 20p |
| مصطلحات موضوعية: |
CHLAMYDIA trachomatis, SEXUALLY transmitted diseases, CARRIER proteins, MEMBRANE proteins, GENITALIA, GENITALIA infections |
| مستخلص: |
Chlamydia trachomatis (Ct) is the most common cause of bacterial sexually transmitted infections (STIs) worldwide. Ct infections are often asymptomatic in women, leading to severe reproductive tract sequelae. Development of a vaccine against Chlamydia is crucial. The Chlamydia major outer membrane protein (MOMP) is a prime vaccine antigen candidate, and it can elicit both neutralizing antibodies and protective CD4+ T cell responses. We have previously designed chimeric antigens composed of immunogenic variable regions (VDs) and conserved regions (CDs) of MOMP from Chlamydia muridarum (Cm) expressed into a carrier protein (PorB), and we have shown that these were protective in a mouse model of Cm respiratory infection. Here, we generated corresponding constructs based on MOMP from Ct serovar F. Preliminary structure analysis of the three antigens, PorB/VD1-3, PorB/VD1-4 and PorB/VD1-2-4, showed that they retained structure features consistent with those of PorB. The antigens induced robust humoral and cellular responses in mice with different genetic backgrounds. The antibodies were cross-reactive against Ct, but only anti-PorB/VD1-4 and anti-PorB/VD1-2-4 IgG antibodies were neutralizing, likely due to the antigen specificity. The cellular responses included proliferation in vitro and production of IFN-γ by splenocytes following Ct re-stimulation. Our results support further investigation of the PorB/VD antigens as potential protective candidates for a Chlamydia subunit vaccine. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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