التفاصيل البيبلوغرافية
| العنوان: |
基于转录组测序分析茯苓多糖对糖尿病 大鼠创面愈合的影响. (Chinese) |
| Alternate Title: |
The effect of Poria cocos polysaccharide on wound healing in diabetic rats analyzed based on RNA-sequencing. (English) |
| المؤلفون: |
彭珍雪, 周芝森, 刘璨宇, 张选奋 |
| المصدر: |
Chinese Journal of Aesthetic & Plastic Surgery; Jul2024, Vol. 37 Issue 7, p425-430, 6p |
| Abstract (English): |
Objective To analyze the effect of poria cocos polysaccharide (PCP) on wound healing in diabetic rats based on RNA-seq. Methods From September to October 2023, 24 SPF SD male rats were randomly divided into diabetic control (DC) group, normal control (NC) group and PCP group (8 rats in each group) in Department of Plastic Surgery, The Second Hospital of Lanzhou Universitv. Previous experiments showed that 1% PCP aqueous solution could promote wound healing in diabetic rats. Wounds in PCP group were treated with 1% PCP aqueous solution on wet compress after operation, while wounds in normal control group and diabetic group were treated with normal saline wet compress. On the 7th day after operation, 5 rats were randomly selected from each group to calculate the wound healing rate, and the wound tissue was cut and stained with HE to observe the pathological changes of the wound tissue in each group. RNA-seq was performed to screen out the significant differential genes in NC, DC group, and DC, PCP group, kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis，gene ontology (Ontology) analysis were performed (GO) enrichment analysis and Gene set enrichment analysis (GSEA) were performed. The remaining 3 rats in each group were photographed and recorded on the 14th day after operation, the wound healing rate was calculated, HE and Masson staining was performed. Results On the 7th and 14th day after injury, the wound of rats in each group was gradually reduced. The wound healing of NC group was the best, DC group was the worst, and PCP group was between DC group and NC group. HE staining showed that there were a large number of inflammatory cells infiltration in DC group and a small number of inflammatory cells infiltration in NC group at the same time point. The inflammatory cells in PCP group were between NC group and DC group. KEGG and GO enrichment analysis showed that the significantly different genes between DC group and NC group were mainly enriched in cAMP, cGMP-PKG and other signaling pathways. It is involved in biological processes such as keratinization, epithelial development, and inflammatory response. The significantly different genes between PCP group and DC group were mainly enriched in PI3K-Akt, Calcium and other signaling pathways. It is involved in the regulation of angiogenesis, epithelial development, and regulation of endothelial cell proliferation and other biological processes. GSEA analysis showed that: The minor differential genes in DC and NC groups were enriched in interleukin-17 (IL-17), nuclear factor nuclear factor kappa-B (NF-κB), Calcium and tumor necrosis factor (TNF) signaling pathways. DC and PCP groups were enriched in IL-17 and cGMP-PKG signaling pathways. Conclusion High glucose may interfere with wound healing in rats through cGMP-PKG signaling pathway; PCP may promote wound healing in diabetic rats by affecting PI3K-Akt and Calcium signaling pathways. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
基于转录组测序分析茯苓多糖 (poria cocos polysaccharide, PCP) 对糖尿病大鼠创面愈合的影响。方法 自 2023 年 9—10 月, 兰州大学第二医院整形外科将 24 只 SPF 级 SD 雄性大鼠, 随机分为糖尿病 (diabetic control, DC) 组、正常对照 (normal control, NC) 组及 PCP 组, 8 只 / 组。前期预实验表明, 1% PCP 水溶液可促进糖尿病大鼠创面愈合。建立创面模型后, PCP 组创面予 1% PCP 水溶液湿敷, NC 组及 DC 组予生理盐水湿敷, 1 次 /d。于术后第 7 天每组各随机抽取 5 只大鼠, 计算创面愈合 率, 切取创面组织, 行 HE 及 Masson 染色, 观察各组大鼠创面组织病理学变化, 行转录组测序 (RNA-sequencing, RNA-seq), 筛 选出 NC、DC 组及 DC、PCP 组显著差异基因, 进行京都基因与基因组百科全书 (kyoto encyclopedia of genes and genomes, KEGG) 富集分析、基因本体 (gene ontology, GO) 富集分析及基因集富集分析 (gene set enrichment analysis, GSEA) 。每组余下各 3 只大鼠 于术后第 14 天拍照记录、计算创面愈合率、行 HE 及 Masson 染色。结果 术后第 7、14 天, 各组大鼠创面逐渐缩小, NC 组创面 愈合情况最佳, DC 组最差, PCP 组介于 DC、NC 组之间。术后第 7、14 天, HE 染色显示, 同一时间点, DC 组有大量炎症细胞浸 润, NC 组有少量炎症细胞浸润, PCP 组介于 NC、DC 组之间, 术后第 14 天各组炎症细胞较术后第 7 天减少。KEGG、GO 富集分 析显示, DC 组与 NC 组差异基因主要富集在 cAMP、cGMP-PKG 等信号通路, 参与角化、上皮发育、炎症反应等生物学过程。PCP 组与 DC 组差异基因主要富集在 PI3K-Akt 、Calcium 等信号通路, 参与新生血管的调控、上皮细胞发育、内皮细胞增殖的调节等 生物学过程。GSEA 分析显示, DC、NC 组微小差异基因富集在白介素 -17 (interleukin-17, IL-17) 、核因子 -资B (nuclear factor kappa-B, NF-资B) 、钙 (Calcium) 、肿瘤坏死因子 (tumor necrosis facto, TNF) 等信号通路, DC、PCP 组富集在 IL-17、cGMP-PKG 等 信号通路。结论 高糖可能通过 cAMP、cGMP-PKG 等信号通路干扰大鼠创面的愈合；PCP 可能通过影响 PI3K-Akt、Calcium 信 号通路促进糖尿病大鼠创面愈合。 [ABSTRACT FROM AUTHOR] |
|
Copyright of Chinese Journal of Aesthetic & Plastic Surgery is the property of Chinese Journal of Aesthetic & Plastic Surgery Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |