التفاصيل البيبلوغرافية
| العنوان: |
Safety of embryo cryopreservation: insights from mid-term placental transcriptional changes. |
| المؤلفون: |
Luo, Qin-Yu, Zhang, Si-Wei, Wu, Hai-Yan, Mo, Jia-Ying, Yu, Jia-En, He, Ren-Ke, Jiang, Zhao-Ying, Zhu, Ke-Jing, Liu, Xue-Ying, Lin, Zhong-Liang, Sheng, Jian-Zhong, Zhang, Yu, Wu, Yan-Ting, Huang, He-Feng |
| المصدر: |
Reproductive Biology & Endocrinology; 7/12/2024, Vol. 22 Issue 1, p1-16, 16p |
| مصطلحات موضوعية: |
FERTILITY preservation, PLACENTA, FETAL growth retardation, EMBRYO implantation, EMBRYOS, GESTATIONAL age |
| مستخلص: |
Background: In recent years, with benefits from the continuous improvement of clinical technology and the advantage of fertility preservation, the application of embryo cryopreservation has been growing rapidly worldwide. However, amidst this growth, concerns about its safety persist. Numerous studies have highlighted the elevated risk of perinatal complications linked to frozen embryo transfer (FET), such as large for gestational age (LGA) and hypertensive disorders during pregnancy. Thus, it is imperative to explore the potential risk of embryo cryopreservation and its related mechanisms. Methods: Given the strict ethical constraints on clinical samples, we employed mouse models in this study. Three experimental groups were established: the naturally conceived (NC) group, the fresh embryo transfer (Fresh-ET) group, and the FET group. Blastocyst formation rates and implantation rates were calculated post-embryo cryopreservation. The impact of FET on fetal growth was evaluated upon fetal and placental weight. Placental RNA-seq was conducted, encompassing comprehensive analyses of various comparisons (Fresh-ET vs. NC, FET vs. NC, and FET vs. Fresh-ET). Results: Reduced rates of blastocyst formation and implantation were observed post-embryo cryopreservation. Fresh-ET resulted in a significant decrease in fetal weight compared to NC group, whereas FET reversed this decline. RNA-seq analysis indicated that the majority of the expression changes in FET were inherited from Fresh-ET, and alterations solely attributed to embryo cryopreservation were moderate. Unexpectedly, certain genes that showed alterations in Fresh-ET tended to be restored in FET. Further analysis suggested that this regression may underlie the improvement of fetal growth restriction in FET. The expression of imprinted genes was disrupted in both FET and Fresh-ET groups. Conclusion: Based on our experimental data on mouse models, the impact of embryo cryopreservation is less pronounced than other in vitro manipulations in Fresh-ET. However, the impairment of the embryonic developmental potential and the gene alterations in placenta still suggested it to be a risky operation. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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