التفاصيل البيبلوغرافية
| العنوان: |
Germ granule compartments coordinate specialized small RNA production. |
| المؤلفون: |
Chen, Xiangyang, Wang, Ke, Mufti, Farees Ud Din, Xu, Demin, Zhu, Chengming, Huang, Xinya, Zeng, Chenming, Jin, Qile, Huang, Xiaona, Yan, Yong-hong, Dong, Meng-qiu, Feng, Xuezhu, Shi, Yunyu, Kennedy, Scott, Guang, Shouhong |
| المصدر: |
Nature Communications; 7/10/2024, Vol. 15 Issue 1, p1-19, 19p |
| مصطلحات موضوعية: |
NON-coding RNA, RNA replicase, CAENORHABDITIS elegans, REGULATOR genes, GERM cells |
| مستخلص: |
Germ granules are biomolecular condensates present in most animal germ cells. One function of germ granules is to help maintain germ cell totipotency by organizing mRNA regulatory machinery, including small RNA-based gene regulatory pathways. The C. elegans germ granule is compartmentalized into multiple subcompartments whose biological functions are largely unknown. Here, we identify an uncharted subcompartment of the C. elegans germ granule, which we term the E granule. The E granule is nonrandomly positioned within the germ granule. We identify five proteins that localize to the E granule, including the RNA-dependent RNA polymerase (RdRP) EGO-1, the Dicer-related helicase DRH-3, the Tudor domain-containing protein EKL-1, and two intrinsically disordered proteins, EGC-1 and ELLI-1. Localization of EGO-1 to the E granule enables synthesis of a specialized class of 22G RNAs, which derive exclusively from 5' regions of a subset of germline-expressed mRNAs. Defects in E granule assembly elicit disordered production of endogenous siRNAs, which disturbs fertility and the RNAi response. Our results define a distinct subcompartment of the C. elegans germ granule and suggest that one function of germ granule compartmentalization is to facilitate the localized production of specialized classes of small regulatory RNAs. Germ granules help to maintain germ cell totipotency. Here, authors identify a distinct subcompartment of the C. elegans germ granule, the E granule, and show that defects in its assembly elicit disordered production of endogenous siRNAs that disturbs fertility and the RNAi response. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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