التفاصيل البيبلوغرافية
| العنوان: |
NaCl 对樱桃砧木组培生根、IAA 原位分布及相关酶活性的影响. (Chinese) |
| Alternate Title: |
Effects of NaCl on rooting, in-situ distribution of IAA and related enzyme activities of cherry rootstock seedlings. (English) |
| المؤلفون: |
高兰芸, 刘昊, 李爱, 张婷婷, 杨丽芳, 高英 |
| المصدر: |
Acta Agriculturae Zhejiangensis; Jun2024, Vol. 36 Issue 6, p1300-1308, 9p |
| Abstract (English): |
In order to clarify the effect of NaCl on the rooting of cherry rootstock, this experiment took the Gisela 6 tissue culture seedling as the material, and firstly studied the index of adventitious root occurrence in the tissue culture seedling treated with different concentrations of NaCl. Then, the anatomical structure, enzyme activity and in-situ distribution of IAA under 10 mmol·L-1and 80 mmol·L-1NaCl were further studied according to rooting indexes. The results showed that the rooting rate and rooting index of cherry tissue seedlings were the highest under the treatment of 10 mmol·L-1NaCl, and were significantly higher than other treatments, which were 58% and 13.43, respectively. Under this treatment the rooting speed was the fastest, the cambium thickened at 2 d, the root primodia formed at 6 d, and the adventitious roots broke through the epidermis at 10 d. SOD activity of each treatment reached the peak at 6 d. The activity changes of POD, PPO and IAAO were different among all treatments, while the activity of CK showed an increasing trend. Compared with CK, the activity of IAAO in 10 mmol·L-1 treatment peaked at 2 d. Using immune colloidal gold technique to analysis the in-situ distribution of IAA, we found under 10 mmol·L-1 NaCl treatment the IAA mainly distributed in cambium at 2 d, in root primordium at 6 d, and in the adventitious root vascular tissue at 10 d. In conclusion, 10 mmol·L-1NaCl treatment can promote adventitious roots of cherry tissue culture seedlings, and provide a reference for further research on the occurrence and regulation mechanism of adventitious roots. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
为阐明 NaCl 对樱桃砧木组培生根的影响, 试验以吉塞拉 6 号组培苗为材料, 首先研究了不同浓度 NaCl 处理下组培苗不定根发生指标, 然后根据生根指标进一步研究了10 mmol·L-1和 80 mmol·L-1NaCl处理下的解剖结构、发根过程酶活性及吲哚-3-2酸 (IAA)的原位分布。结果表明, 10 mmol·L-1NaCl处理下樱桃组培苗生根率和生根指数均最高, 且显著高于其他处理, 分别为58%和13.43; 该处理下生根最快, 2 d时形成层开始增厚, 6 d时根原基形成, 10 d时不定根突破表皮。各处理超氧化物歧化酶 (SOD)活性在6 d时均达到峰值; 过氧化物酶 (POD)、多酚氧化酶 (PPO)、吲哚乙酸氧化酶 (IAAO)活性变化各处理存在差异, CK均呈上升趋势, 与 CK 相比, 10 mmol·L-1处理IAAO活性于 2 d出现峰值。利用免疫胶体金技术对IAA进行原位分析发现, 10 mmol·L-1处理2 d 时 IAA 主要分布于形成层, 6 d 时 IAA 大量分布于根原基, 10 d时I AA 集中分布于不定根维管组织。综上, 10 mmol·L-1NaCl处理对樱桃组培苗不定根发生具有一定的促进作用, 为进一步研究樱桃组培苗不定根发生及调控机制提供参考。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |