التفاصيل البيبلوغرافية
| العنوان: |
Response surface methodology optimization extraction of polysaccharide from Tricholoma lobayense and its biological activities. |
| المؤلفون: |
Moorthy, Aswini, Govindan, Sudha, Janardhanan, Amal, Srinivasan, Sachindev, Padinjarathil, Himabindu, Ramani, Prasanna |
| المصدر: |
Journal of Food Measurement & Characterization; Mar2024, Vol. 18 Issue 3, p2133-2145, 13p |
| مصطلحات موضوعية: |
RESPONSE surfaces (Statistics), POLYSACCHARIDES, URONIC acids, IRON ions, HYDROXYL group, PHAGOCYTOSIS |
| مستخلص: |
The extraction of polar polysaccharides from the sporocarp of Trichicholoma lobayense (TLP) was optimised using a response surface approach. The extraction processing factors were optimised using RSM with Box–Behnken design (BBD). The ideal extraction conditions are as follows: 94.32 °C for the extraction temperature, 1.92 h for the extraction time, and 10.32 mL/g for the liquid-to-solid ratio. An experimental yield of 10.82%, which agrees well with the anticipated yield, was produced under the optimised circumstances. With a comparatively high concentration of uronic acids (14.23%), TLP had a protein and carbohydrate content of 3.36% and 79.50%, respectively. TLP showed potent in vitro antioxidant scavenging activity for DPPH, hydroxyl radicals, and cupric ions but only modest chelating ability, β-carotene bleaching inhibition, and ferric ion reduction power. The inhibitory effects of TLP against L929 fibroblast and B16-F10 murine metastatic melanoma cells were 72.21% and 77.07%, respectively, at a dose of 100 µg/mL. TLP enhanced macrophage phagocytosis, demonstrating immunomodulatory action. The results of this work will lay the basis for understanding the effective extraction of TLP, which may then be investigated as a possible antioxidant, anticancer, and immunomodulating agent. [ABSTRACT FROM AUTHOR] |
|
Copyright of Journal of Food Measurement & Characterization is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |