التفاصيل البيبلوغرافية
| العنوان: |
An Observation of a Very High Swelling of Bromovirus Members at Specific Ionic Strengths and pH. |
| المؤلفون: |
Segovia-González, Xochitl Fabiola, Villagrana-Escareño, Maria Veronica, Ríos-Ramírez, Maricarmen, de la Cruz, Vianey Santiago, Mejía-Hernández, Jessica Nathaly, Cuellar-Camacho, Jose Luis, Patrón-Soberano, Araceli, Sportsman, Richard, Ruiz-García, Jaime |
| المصدر: |
Viruses (1999-4915); Oct2023, Vol. 15 Issue 10, p2046, 14p |
| مصطلحات موضوعية: |
IONIC strength, ATOMIC force microscopy, TRANSMISSION electron microscopy, PLANT viruses, MOSAIC viruses, LIGHT scattering |
| مستخلص: |
Cowpea chlorotic mottle virus (CCMV) and brome mosaic virus (BMV) are naked plant viruses with similar characteristics; both form a T = 3 icosahedral protein capsid and are members of the bromoviridae family. It is well known that these viruses completely disassemble and liberate their genome at a pH around 7.2 and 1 M ionic strength. However, the 1 M ionic strength condition is not present inside cells, so an important question is how these viruses deliver their genome inside cells for their viral replication. There are some studies reporting the swelling of the CCMV virus using different techniques. For example, it is reported that at a pH~7.2 and low ionic strength, the swelling observed is about 10% of the initial diameter of the virus. Furthermore, different regions within the cell are known to have different pH levels and ionic strengths. In this work, we performed several experiments at low ionic strengths of 0.1, 0.2, and 0.3 and systematically increased the pH in 0.2 increments from 4.6 to 7.4. To determine the change in virus size at the different pHs and ionic strengths, we first used dynamic light scattering (DLS). Most of the experiments agree with a 10% capsid swelling under the conditions reported in previous works, but surprisingly, we found that at some particular conditions, the virus capsid swelling could be as big as 20 to 35% of the original size. These measurements were corroborated by atomic force microscopy (AFM) and transmission electron microscopy (TEM) around the conditions where the big swelling was determined by DLS. Therefore, this big swelling could be an easier mechanism that viruses use inside the cell to deliver their genome to the cell machinery for viral replication. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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