التفاصيل البيبلوغرافية
| العنوان: |
单轴及同轴载盐酸米诺环素骨支架抑菌性能的评价. (Chinese) |
| Alternate Title: |
Evaluation of antibacterial properties of uniaxial and coaxial minocycline hydrochloride-loaded bone scaffolds. (English) |
| المؤلفون: |
曹怡静, 魏岁艳, 赵 帅, 李冬瑶, 魏 琴, 张旭婧, 许 燕, 徐国强 |
| المصدر: |
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu; 4/8/2024, Vol. 28 Issue 10, p1547-1553, 7p |
| مصطلحات موضوعية: |
BONE grafting, PORPHYROMONAS gingivalis, TISSUE scaffolds, RAPID prototyping, FILTER paper, BONE growth |
| مصطلحات جغرافية: |
CHINA |
| Abstract (English): |
BACKGROUND: Due to the unstable drug release rate of uniaxial bone scaffolds, multi-structure composite printing methods have been sought in and outside China in recent years. Currently, coaxial drug-loaded bone scaffolds, which combine drug-loaded sustained release system with bone transplantation and repair technology, not only replace the defective bone after implantation, but also release drugs slowly, providing a microenvironment conducive to bone formation at the implant site. OBJECTIVE: To explore and assess the in vitro antibacterial properties of uniaxial and coaxial minocycline hydrochloride bone scaffolds. METHODS: Rapid prototyping technology was used to prepare uniaxial hydroxyapatite/silk fibroin-polyvinyl alcohol scaffold, uniaxial hydroxyapatite/silk fibroin-polyvinyl alcohol scaffold, coaxial hydroxyapatite/silk fibroin-polyvinyl alcohol scaffold, and coaxial hydroxyapatite/silk fibroin-polyvinyl alcohol scaffold, respectively, which were named S1, S2, T1 and T2. The morphology, porosity, degradation performance, in vitro sustained-release performance and cytotoxicity of scaffolds were characterized. Four kinds of bone scaffolds were immersed in PBS to prepare the extracts at different time points (1, 3, 5, 7, 14, 21, and 28 days). The qualitative filter paper was placed into the extract for 24 hours. The filter paper was co-cultured with Porphyromonas gingivalis and Fusobacterium nucleatum for 72 hours. The bacteriostatic effect of four groups of scaffolds was detected by the agar diffusion method. RESULTS AND CONCLUSION: (1) Scaffold characterization: Four groups of scaffolds were well formed. The surface of micro-wires in the S1 and S2 groups was dense and smooth, and the surface of micro-wires in the T1 and T2 groups was rough. Porosity was between 40%-47% and met the requirements of bone scaffolds. Compared with the S2 group, sustained release time was longer in the T2 group. The sustained release concentration of the drug was between 1- 10 μg/mL for a long time, which was more conducive to bacteriostasis and osteogenesis. After 10 weeks of immersion in PBS in vitro, the degradation rate of the coaxial printed bone scaffold was faster than that of the corresponding uniaxial printed bone scaffold, and the degradation rate of the coaxial loaded bone scaffold was lower than that of the coaxial non-loaded bone scaffold. The four groups of scaffold extracts were co-cultured with osteoblasts respectively. CCK-8 assay displayed that the cell proliferation rate was greater than 75%, which met the requirements of biocompatibility. (2) In vitro antibacterial effect: S1 and T1 did not have antibacterial activity. S2 and T2 had an obvious antibacterial effect. Under the extraction solution on day 28, the diameter of Porphyromonas gingivalis and Fusobacterium nucleatum inhibition zone in the S2 group was smaller than that in the T2 group (P < 0.05). (3) These findings exhibit that hydroxyapatite/silk fibroin-polyvinyl alcohol scaffolds with coaxial minocycline have good physical properties and bacteriostatic properties. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
背景：由于单轴骨支架的药物缓释速率不稳定，近年来国内外开始寻求多结构复合型打印方式，目前有将载药缓释系统与骨移植修复技术 相结合的同轴载药骨支架，其在植入体内后不仅替代了缺损骨，还可缓慢释放药物，为植入部位提供一个利于成骨的微环境。 目的：探讨并评价单轴及同轴载盐酸米诺环素骨支架的体外抑菌性能。 方法：采用快速成型技术分别制备单轴羟基磷灰石/丝素蛋白-聚乙烯醇支架、单轴载米诺环素的羟基磷灰石/丝素蛋白-聚乙烯醇支架、 同轴羟基磷灰石/丝素蛋白-聚乙烯醇支架、同轴载米诺环素的羟基磷灰石/丝素蛋白-聚乙烯醇支架，分别命名为S1、S2、T1、T2，表征支 架的形态、孔隙率、降解性能、体外缓释性能及细胞毒性。将4种骨支架浸泡于PBS中制备不同时间点(1，3，5，7，14，21，28 d)的浸提 液，再将定性滤纸片置入浸提液中浸提24 h，将滤纸片分别与牙龈卟啉单胞菌、具核梭酸杆菌共培养72 h，采用琼脂扩散法检测4组支架的 抑菌作用。 结果与结论：①支架表征：4 组支架均成型良好，扫描电镜下可见，S1、S2组微观丝材表面致密且光滑，T1、T2组微观丝材表面呈现粗糙 状，孔隙率大小在40%-47%之间，均满足骨支架的要求；与S2比较，T2的缓释时间更长，且药物缓释浓度长期处于1-10 μg/mL之间，更有 利于抑菌及成骨；体外浸泡于PBS中10周，同轴打印骨支架的降解速率快于对应的单轴打印骨支架，并且同轴载药支架的降解率低于同轴 不载药组；将4组支架浸提液分别与成骨细胞共培养，CCK-8检测显示细胞增殖率均大于75%，均满足生物相容性要求；②支架抑菌作用： S1、T1不具有抑菌性能，S2、T2具有抑菌性能，在第28天的浸提液下，S2组对牙龈卟啉单胞菌、具核梭酸杆菌的抑菌圈直径均小于T2组 (P < 0.05)；③结果表明：同轴载米诺环素的羟基磷灰石/丝素蛋白-聚乙烯醇支架具有良好的物理性能及抑菌性能。 [ABSTRACT FROM AUTHOR] |
|
Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |