التفاصيل البيبلوغرافية
| العنوان: |
UBL3 Interacts with Alpha-Synuclein in Cells and the Interaction Is Downregulated by the EGFR Pathway Inhibitor Osimertinib. |
| المؤلفون: |
Chen, Bin, Hasan, Md. Mahmudul, Zhang, Hengsen, Zhai, Qing, Waliullah, A. S. M., Ping, Yashuang, Zhang, Chi, Oyama, Soho, Mimi, Mst. Afsana, Tomochika, Yuna, Nagashima, Yu, Nakamura, Tomohiko, Kahyo, Tomoaki, Ogawa, Kenji, Kaneda, Daita, Yoshida, Minoru, Setou, Mitsutoshi |
| المصدر: |
Biomedicines; Jun2023, Vol. 11 Issue 6, p1685, 16p |
| مصطلحات موضوعية: |
EPIDERMAL growth factor receptors, LUCIFERASES, ALPHA-synuclein, OSIMERTINIB, CYSTEINE, POST-translational modification, EXTRACELLULAR vesicles |
| مستخلص: |
Ubiquitin-like 3 (UBL3) acts as a post-translational modification (PTM) factor and regulates protein sorting into small extracellular vesicles (sEVs). sEVs have been reported as vectors for the pathology propagation of neurodegenerative diseases, such as α-synucleinopathies. Alpha-synuclein (α-syn) has been widely studied for its involvement in α-synucleinopathies. However, it is still unknown whether UBL3 interacts with α-syn, and is influenced by drugs or compounds. In this study, we investigated the interaction between UBL3 and α-syn, and any ensuing possible functional and pathological implications. We found that UBL3 can interact with α-syn by the Gaussia princeps based split luciferase complementation assay in cells and immunoprecipitation, while cysteine residues at its C-terminal, which are considered important as PTM factors for UBL3, were not essential for the interaction. The interaction was upregulated by 1-methyl-4-phenylpyridinium exposure. In drug screen results, the interaction was significantly downregulated by the treatment of osimertinib. These results suggest that UBL3 interacts with α-syn in cells and is significantly downregulated by epidermal growth factor receptor (EGFR) pathway inhibitor osimertinib. Therefore, the UBL3 pathway may be a new therapeutic target for α-synucleinopathies in the future. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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