التفاصيل البيبلوغرافية
| العنوان: |
Solubility and Thermal Stability of Thermotoga maritima MreB. |
| المؤلفون: |
Longauer, Beáta, Bódis, Emőke, Lukács, András, Barkó, Szilvia, Nyitrai, Miklós |
| المصدر: |
International Journal of Molecular Sciences; Dec2022, Vol. 23 Issue 24, p16044, 13p |
| مصطلحات موضوعية: |
THERMOTOGA maritima, THERMAL stability, SOLUBILITY, CRYSTAL structure, ADENOSINE triphosphate, POLYMERIZATION, TRYPTOPHAN |
| مستخلص: |
The basis of MreB research is the study of the MreB protein from the Thermotoga maritima species, since it was the first one whose crystal structure was described. Since MreB proteins from different bacterial species show different polymerisation properties in terms of nucleotide and salt dependence, we conducted our research in this direction. For this, we performed measurements based on tryptophan emission, which were supplemented with temperature-dependent and chemical denaturation experiments. The role of nucleotide binding was studied through the fluorescent analogue TNP-ATP. These experiments show that Thermotoga maritima MreB is stabilised in the presence of low salt buffer and ATP. In the course of our work, we developed a new expression and purification procedure that allows us to obtain a large amount of pure, functional protein. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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