التفاصيل البيبلوغرافية
| العنوان: |
Laser and LED photobiomodulation effects in osteogenic or regular medium on rat calvaria osteoblasts obtained by newly forming bone technique. |
| المؤلفون: |
Cardoso, Matheus Völz, do Vale Placa, Rebeca, Sant'Ana, Adriana Campos Passanezi, Greghi, Sebastião Luiz Aguiar, Zangrando, Mariana Schutzer Ragghianti, de Rezende, Maria Lucia Rubo, Oliveira, Rodrigo Cardoso, Damante, Carla Andreotti |
| المصدر: |
Lasers in Medical Science; Apr2021, Vol. 36 Issue 3, p541-553, 13p |
| مصطلحات موضوعية: |
PHOTOBIOMODULATION therapy, BONE growth, OSTEOBLASTS, CALVARIA, ALKALINE phosphatase, BONE density |
| مستخلص: |
The purposes of this study are to evaluate the effects of photobiomodulation (PBM) with laser and LED on rat calvaria osteoblasts (rGO lineage), cultured in osteogenic (OST) or regular (REG) medium, after induction of a quiescent state and to test if PBM is capable of osteogenic induction and if there is a sum of effects when combining OST medium with PBM. Before irradiation, the cells were put in a quiescent state (1% FBS) 24 h, when red (AlGaInP—660 nm) and infrared laser (GaAlAs—808 nm) and LED (637 ± 15 nm) were applied. The groups were as follows: red laser (RL3—5 J/cm2, 3 s and RL5—8.3 J/cm2, 5 s, 1.66 W/cm2); infrared laser (IrL3—5 J/cm2, 3 s and IrL5–8.3 J/cm2, 5 s); LED (LED3—3 s and LED5—5 s, 0.02 J/cm2, 0.885 W/cm2); positive (C+, 10% FBS) and negative control (C−, 1% FBS). For alkaline phosphatase (ALP) and mineralization assays, the cells were cultured in REG (DMEM 10% FBS) and OST medium (DMEM 10% FBS, 50 μg/mL ascorbic acid, 10 mM β-glycerophosphate). Statistical analysis was performed using ANOVA and Tukey's tests (p < 0.05). RL5 and LED5 increased proliferation, in vitro wound closure, ALP, and mineralization in rGO cells (p < 0.05). PBM with red laser and LED induced mineralization by itself, without osteogenic medium, not observed for infrared laser (p < 0.05). A sum of effects was observed in osteogenic medium and PBM by infrared, red laser, and LED (5 s). Red laser and LED increased proliferation, migration, and secretory phases in rGO cells in a dose-dependent manner. PBM with red laser and LED promotes osteogenic induction by itself. PBM with infrared laser and osteogenic medium potentializes mineralization. [ABSTRACT FROM AUTHOR] |
|
Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |