التفاصيل البيبلوغرافية
| العنوان: |
A human monoclonal antibody blocks malaria transmission and defines a highly conserved neutralizing epitope on gametes. |
| المؤلفون: |
Coelho, Camila H., Tang, Wai Kwan, Burkhardt, Martin, Galson, Jacob D., Muratova, Olga, Salinas, Nichole D., Alves e Silva, Thiago Luiz, Reiter, Karine, MacDonald, Nicholas J., Nguyen, Vu, Herrera, Raul, Shimp, Richard, Narum, David L., Byrne-Steele, Miranda, Pan, Wenjing, Hou, Xiaohong, Brown, Brittany, Eisenhower, Mary, Han, Jian, Jenkins, Bethany J. |
| المصدر: |
Nature Communications; 3/19/2021, Vol. 12 Issue 1, p1-12, 12p |
| مصطلحات موضوعية: |
MONOCLONAL antibodies, GAMETES, B cell receptors, MALARIA, CYTOSKELETAL proteins, MALARIA vaccines, VACCINATION |
| مستخلص: |
Malaria elimination requires tools that interrupt parasite transmission. Here, we characterize B cell receptor responses among Malian adults vaccinated against the first domain of the cysteine-rich 230 kDa gamete surface protein Pfs230, a key protein in sexual stage development of P. falciparum parasites. Among nine Pfs230 human monoclonal antibodies (mAbs) that we generated, one potently blocks transmission to mosquitoes in a complement-dependent manner and reacts to the gamete surface; the other eight show only low or no blocking activity. The structure of the transmission-blocking mAb in complex with vaccine antigen reveals a large discontinuous conformational epitope, specific to domain 1 of Pfs230 and comprising six structural elements in the protein. The epitope is conserved, suggesting the transmission-blocking mAb is broadly functional. This study provides a rational basis to improve malaria vaccines and develop therapeutic antibodies for malaria elimination. Vaccines that interrupt malaria transmission will be important tools for malaria elimination. Here the authors identify a human monoclonal antibody from Pfs230 vaccinated individuals that blocks transmission of Plasmodium falciparum to mosquitoes in a complement-dependent manner and reacts with gamete surface. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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