التفاصيل البيبلوغرافية
| العنوان: |
UPDATING THE ROUTINE SPECIATION OF ROMANIAN CAMPYLOBACTER STRAINS. |
| المؤلفون: |
Militaru, Mădălina, Sorokin, Marilena, Ciontea, Simona Adriana, Cristea, Daniela, Usein, Codruţa-Romaniţa, Stoica, Ileana |
| المصدر: |
Romanian Archives of Microbiology & Immunology; Jul-Sep2019, Vol. 78 Issue 3, p145-148, 4p |
| Abstract (English): |
Introduction. Diarrhea caused by Campylobacter spp., especially Campylobacter jejuni and Campylobacter coli, is one of the most frequently reported gastrointestinal infection raising public health concern in many European countries. Objectives. In order to improve the speciation protocol of Campylobacter strains for optimal disease surveillance, a PCR approach was introduced for species specific detection of C. jejuni and C. coli at reference laboratory level. Methods. A collection of 70 Campylobacter strains previously identified at species level based on the hippurate hydrolysis biochemical test was used to test a three-gene multiplex-PCR-based method. previously published primers were used to target the hippuricase gene (hipO) characteristic of C. jejuni, aspartokinase gene (asp) characteristic of C. coli, and a universal 16s rdna gene sequence serving as an internal positive control. Results. The phenotypic and genotypic methods were found to be 90% in concordance as six strains initially identified as C. coli by the biochemical tests proved to be C. jejuni in the PCR tests. Conclusion. The established multiplex PCR approach can be used for routine diagnosis as well as epidemiological purposes. [ABSTRACT FROM AUTHOR] |
| Abstract (Romanian): |
Introducere. Diareea produsă de speciile Campylobacter jejuni si Campylobacter coli este printre cele mai frecvente infecții gastrointestinale, care ridică probleme de sănătate publică în multe țări europene. Obiective. Pentru a îmbunătăți identificarea speciilor de Campylobacter, necesară pentru supravegherea optimă a infecției, la nivelul laboratorului de referință a fost introdus un protocol de analiză bazat pe PCR. Metode. O colecție formată din 70 tulpini de Campylobacter, inițial identificate la nivel de specie cu ajutorul testului de hidroliză a hipuratului, a fost utilizată pentru a pune la punct un protocol bazat pe reacția PCR multiplex. S-au folosit primeri preluați din literatură, pentru amplificarea genei pentru hipuricază (hipo), caracteristică speciei C. jejuni, genei pentru aspartokinază (asp), specifică pentru C. coli şi genei pentru ARNr 16s, care a servit drept control intern. Rezultate. Concordanța dintre metoda fenotipică şi genotipică a fost de 90%, şase dintre tulpinile identificate biochimic drept C.coli dovedindu-se a fi, la testarea prin PCR, C. jejuni. Concluzie. Protocolul bazat pe PCR multiplex poate fi aplicat atât în scop diagnostic, cât şi epidemiologic. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |