التفاصيل البيبلوغرافية
| العنوان: |
AllergoOncology: Expression platform development and functional profiling of an anti‐HER2 IgE antibody. |
| المؤلفون: |
Ilieva, Kristina M., Fazekas‐Singer, Judit, Bax, Heather J., Crescioli, Silvia, Montero‐Morales, Laura, Mele, Silvia, Sow, Heng Sheng, Stavraka, Chara, Josephs, Debra H., Spicer, James F., Steinkellner, Herta, Jensen‐Jarolim, Erika, Tutt, Andrew N. J., Karagiannis, Sophia N. |
| المصدر: |
Allergy; Oct2019, Vol. 74 Issue 10, p1985-1989, 5p, 2 Graphs |
| مصطلحات موضوعية: |
IMMUNOGLOBULINS, MEDICAL sciences, IMMUNOGLOBULIN E, ALLERGIC conjunctivitis, EXPERIMENTAL medicine, BLOOD cells, MEDICAL research |
| مستخلص: |
Building upon ours and others' previous methodologies, we report the efficient transient expression and functional evaluation of IgE, exemplified using the variable region sequences of trastuzumab and human IgE constant regions (anti-HER2 IgE). Like trastuzumab, anti-HER2 IgE recognized HER2/ I neu i -overexpressing (BT-474, ZR75-30) breast cancer cells and moderately expressing MCF-10 normal breast cells, and its HER2 antigen recognition kinetic profile on tumour cells was comparable to trastuzumab (Figure A). Importantly, the lack of anti-HER2 IgE blood basophil activation points to diminishing potential safety concerns associated with using IgE class antibodies in cancer immunotherapy. Harnessing engineered antibodies of the IgE class to combat malignancy: initial assessment of FcvarepsilonRI-mediated basophil activation by a tumour-specific IgE antibody to evaluate the risk of type I hypersensitivity. [Extracted from the article] |
|
Copyright of Allergy is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |