التفاصيل البيبلوغرافية
| العنوان: |
In Vitro Study of DNA Adduct 8-hidroxy-2'-deoxyguanosine (8-OHdG) Formation Through Fenton-like Reaction with Butylated Hydroxytoluene Quinone (BHT Quinone). |
| المؤلفون: |
Budiawan, B., Handayani, S., Dani, I. C., Bakri, R., Hannaa, S., Irawati, F. |
| المصدر: |
AIP Conference Proceedings; 2018, Vol. 2023 Issue 1, p020060-1-020060-4, 4p |
| مصطلحات موضوعية: |
DNA adducts, DEOXYGUANOSINE, DNA damage, HABER-Weiss reaction, REVERSE phase liquid chromatography, HIGH performance liquid chromatography |
| مستخلص: |
In this research, in vitro study of DNA adduct 8-hidroxy-2'-deoxyguanosine (8-OHdG) formation as biomarkers of DNA damage was conducted by reacting 2'-deoxyguanosine (2'dG) with BHT-Q through Fenton-like reaction (Cr(III) and H2O2). The conditions of reaction were varied in pH (7.4 and pH 8.4), temperature (37 °C and 60 °C) and incubation time (7 and 12 hours). The 8-OHdG produced was analyzed by using a reverse phase HPLC with UVVis detector. The result showed that 8-OHdG produced from reaction between 2'-dG, BHT-Q, in the presence of Cr(III), and H2O2 was the highest (Fenton-like reaction). While, at a temperature of 60 °C for 12 hours incubation time at pH 7.4 and 8.4, DNA adduct 8-OHdG formation was detected at each of 9.145 ppb and 10.601 ppb. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |