التفاصيل البيبلوغرافية
| العنوان: |
Nuclei migrate through constricted spaces using microtubule motors and actin networks in C. elegans hypodermal cells. |
| المؤلفون: |
Bone, Courtney R., Yu-Tai Chang, Cain, Natalie E., Murphy, Shaun P., Starr, Daniel A. |
| المصدر: |
Development (09501991); 11/15/2016, Vol. 143 Issue 22, p4193-4202, 10p |
| مصطلحات موضوعية: |
MICROTUBULES, CAENORHABDITIS elegans genetics, ACTIN |
| مستخلص: |
Cellular migrations through constricted spaces are a crucial aspect of many developmental and disease processes includinghematopoiesis, inflammation and metastasis. A limiting factor in these events is nuclear deformation. Here, we establish an in vivo model in which nuclei can be visualized while moving through constrictions and use it to elucidatemechanisms for nuclear migration. C. elegans hypodermal P-cell larval nuclei traverse a narrowspace that is about5%their width. This constriction is blocked by fibrous organelles, structures that pass through P cells to connect the muscles to cuticle. Fibrous organelles are removed just prior to nuclear migration, when nuclei and lamins undergo extreme morphological changes to squeeze through the space. Both actin and microtubule networks are organized to mediate nuclear migration. The LINC complex, consisting of the SUN protein UNC-84 and the KASH protein UNC-83, recruits dynein and kinesin-1 to the nuclear surface. Bothmotors function in P-cell nuclear migration, but dynein, functioning through UNC-83, plays a more central role as nuclei migrate towards minus ends of polarized microtubule networks. Thus, the nucleoskeleton and cytoskeleton are coordinated to move nuclei through constricted spaces. [ABSTRACT FROM AUTHOR] |
|
Copyright of Development (09501991) is the property of Company of Biologists Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder