التفاصيل البيبلوغرافية
| العنوان: |
Evidence That an Endothelial Cytosolic Protein Binds to the 3′-Untranslated Region of Endothelial Nitric Oxide Synthase mRNA. |
| المؤلفون: |
De Miguel, Lourdes Sánchez, Alonso, Javier, González-Fernández, Fernando, De La Osada, Jesús, Montón, Mercedes, Rodríguez-Feo, Juan A., Guerra, José I., Arriero, María M., Rico, Luis, Casado, Santos, López-Farré, Antonio |
| المصدر: |
Journal of Vascular Research; 1999, Vol. 36 Issue 3, p201-208, 8p, 7 Diagrams |
| مصطلحات موضوعية: |
NITRIC-oxide synthases, ENDOTHELIUM, CARDIOVASCULAR diseases, MESSENGER RNA, PROTEINS |
| مستخلص: |
Changes in the endothelial nitric oxide synthase (eNOS) expression could be involved in the endothelium-dependent vasorelaxing dysfunction associated with cardiovascular diseases. We have recently demonstrated the existence of endothelial cytosolic proteins that bind to the 3′-untranslated region (3′-UTR) of eNOS mRNA and could be involved in eNOS mRNA stabilization. In the present work, we have characterized the cytosolic proteins that bind to 3′-UTR eNOS mRNA. An endothelial cytosolic protein (MW 60-kD) specifically bound to 3′-UTR eNOS mRNA as determined by a cross-linking assay followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The endothelial cytosolic protein recognized a cytidine (C)-rich region within 3′-UTR eNOS mRNA. Furthermore, tumor necrosis factor-α (TNF-α) increased the level of the 60-kD endothelial cytosolic protein. In addition, TNF-α reduced eNOS mRNA levels and this was prevented by coincubation with cycloheximide. Cycloheximide also prevented the binding activity of the endothelial cytosolic protein to 3′-UTR eNOS mRNA. In summary, these data suggest that a 60-kD endothelial cytosolic protein binds to 3′-UTR eNOS mRNA. TNF-α increased the 60-kD protein levels. Cycloheximide prevented the binding activity of the cytosolic protein to 3′-UTR eNOS mRNA related to TNF-α; this effect was associated with greater eNOS mRNA levels. Further specific studies are needed to determine the involvement of this 60-kD endothelial cytosolic protein in the regulation of eNOS mRNA stabilization and in the endothelial dysfunction associated with cardiovascular diseases. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |