التفاصيل البيبلوغرافية
| العنوان: |
DeepEnzyme: a robust deep learning model for improved enzyme turnover number prediction by utilizing features of protein 3D-structures. |
| المؤلفون: |
Wang, Tong1,2 (AUTHOR), Xiang, Guangming1 (AUTHOR), He, Siwei1 (AUTHOR), Su, Liyun2 (AUTHOR), Wang, Yuguang3,4 (AUTHOR), Yan, Xuefeng5,6 (AUTHOR), Lu, Hongzhong1 (AUTHOR) hongzhonglu@sjtu.edu.cn |
| المصدر: |
Briefings in Bioinformatics. Sep2024, Vol. 25 Issue 5, p1-10. 10p. |
| مصطلحات موضوعية: |
*TRANSFORMER models, TURNOVER frequency (Catalysis), BIOENGINEERING, PROTEIN engineering, SYNTHETIC proteins |
| مستخلص: |
Turnover numbers (k cat), which indicate an enzyme's catalytic efficiency, have a wide range of applications in fields including protein engineering and synthetic biology. Experimentally measuring the enzymes' k cat is always time-consuming. Recently, the prediction of k cat using deep learning models has mitigated this problem. However, the accuracy and robustness in k cat prediction still needs to be improved significantly, particularly when dealing with enzymes with low sequence similarity compared to those within the training dataset. Herein, we present DeepEnzyme, a cutting-edge deep learning model that combines the most recent Transformer and Graph Convolutional Network (GCN) to capture the information of both the sequence and 3D-structure of a protein. To improve the prediction accuracy, DeepEnzyme was trained by leveraging the integrated features from both sequences and 3D-structures. Consequently, DeepEnzyme exhibits remarkable robustness when processing enzymes with low sequence similarity compared to those in the training dataset by utilizing additional features from high-quality protein 3D-structures. DeepEnzyme also makes it possible to evaluate how point mutations affect the catalytic activity of the enzyme, which helps identify residue sites that are crucial for the catalytic function. In summary, DeepEnzyme represents a pioneering effort in predicting enzymes' k cat values with improved accuracy and robustness compared to previous algorithms. This advancement will significantly contribute to our comprehension of enzyme function and its evolutionary patterns across species. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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