التفاصيل البيبلوغرافية
| العنوان: |
Changes in glucose-6-phosphate dehydrogenase expression results in altered behavior of HBV-associated liver cancer cells. |
| المؤلفون: |
Huaidong Hu1,2,3, Xiangchun Ding1,4, Yixuan Yang1, Hongmin Zhang1, Hong Li1, Shiwen Tong1, Xuan An1, Qing Zhong1, Xiaoyan Liu4, Lina Ma4, Qing Liu5, Bin Liu5, Zhenhui Lu5, Dazhi Zhang1,2,3, Peng Hu1,2,3, Hong Ren1,2,3 renhong0531@vip.sina.com |
| المصدر: |
American Journal of Physiology: Gastrointestinal & Liver Physiology. Sep2014, Vol. 307 Issue 6, pG611-G622. 12p. |
| مصطلحات موضوعية: |
*LIVER cancer, *HEPATITIS B virus, *RNA interference, *CELL culture, *CANCER invasiveness, *GLYCOGENOLYSIS, *CARCINOGENESIS |
| مستخلص: |
Hepatocellular carcinoma (HCC) is regarded as a major global health care issue, and chronic hepatitis B virus (HBV) infection is considered to be involved in pathogenesis of HCC. To increase knowledge of HCC pathogenesis, as well as discover potential novel molecules for anti-cancer therapy, mass spectrometry and isobaric tag for relative and absolute quantitation (iTARQ) were employed. The differences between nine HBV-related HCC and adjacent non-HCC tissue specimens were studied. In total, 222 proteins were analyzed for differential expression in the two types of samples. Among these proteins, several were further confirmed by immunohistochemical, immunoblotting, and real-time RT-PCR analysis. RNA interference induced downregulation of glucose-6-phosphate dehydrogenase (G6PD) and decreased HBV replication by fivefold by the IFN pathway. Decreased G6PD expression resulted in decreased hepatoma cell migration and invasion in cell culture. In summary, the investigation provides new information on pathogenesis of HBV infection and suggests G6PD as a novel anti-HCC target. G6PD suppression may contribute to treatment strategies for inhibiting tumor progression. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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