التفاصيل البيبلوغرافية
| العنوان: |
美沙拉嗪通过Wnt1 通路改善溃疡性结肠炎小鼠结肠屏障功能的机制研究. |
| Alternate Title: |
Mechanism of mesalazine ameliorating colonic barrier function in mice with ulcerative colitis through Wnt1 pathway. |
| المؤلفون: |
秦小红1, 王梦远2 wmyuan8899@163.com |
| المصدر: |
Chinese Journal of Immunology. Dec2024, Vol. 40 Issue 12, p2500-2505. 6p. |
| مصطلحات موضوعية: |
*MOUSE mammary tumor virus, *ULCERATIVE colitis, *DEXTRAN sulfate, *SODIUM sulfate, *B cells |
| Abstract (English): |
Objective: To investigate effect of mesalazine (MSLZ) on improvement of colonic barrier in mice with ulcerative colitis(UC), and its regulatory mechanism on wingless-type mouse mammary tumor virus integration site1(Wnt1) signaling pathway. Methods: C57BL/6 mice were randomly divided into normal control group(Control), model group(UC), MSLZ group and MSLZ+sh-Wnt1 group. Except for Control group, other groups were treated with sodium dextran sulfate (DSS) to establish UC model. Mice in Control, UC and MSLZ groups were intraperitoneally injected 5×109 pfu/ml NC-shRNA. Mice in MSLZ+sh-Wnt1 group were intraperitoneally injected 5×109 pfu/ml shRNA-Wnt1. Mice in MSLZ group and MSLZ+sh-Wnt1 group were gavaged with 400 mg/kg MSLZ every day, and mice in Control and UC groups were gavaged with sterile distilled water of same dose every day. Colon length of mice in each group was detected. Kit was used to detect contents of D-lactate (D-LA), lipopolysaccharide (LPS) and activity of diamine oxidase (DAO) in serum of mice in each group. TUNEL staining was used to detect apoptosis in colon tissue of mice in each group. Immunohistochemistry was used to detect expressions of mucin 2 (MUC2) and Occludin in colon tissues. Western blot was used to detect expressions of Wnt1, β-catenin, B cell lymphoma-2(Bcl-2) and Bcl-2 associated X protein(Bax). Results: MSLZ could significantly increase length of colon, increase expressions of MUC2, Occludin, Wnt1, β-catenin and Bcl-2 in colon tissue, decrease levels of D-LA, LPS, DAO in serum, cell apoptosis rate and expression of Bax in colon tissue, while MSLZ+sh-Wnt1 could partially reverse protective effect of MSLZ on colon barrier function (all P<0.05). Conclusion: MSLZ can inhibit apoptosis of colon mucosa in UC mice, alleviate pathological damage of colon tissue, and ameliorate barrier function of colon mucosa, which may be related to activation of Wnt1 signaling pathway. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
目的: 探讨美沙拉嗪（MSLZ）对溃疡性结肠炎（UC）小鼠结肠屏障的改善作用及其对无翅型小鼠乳房肿瘤病 毒整合位点1（Wnt1）信号的调控机制。方法: C57BL/6小鼠随机分为正常对照组（Control）、模型组（UC）、MSLZ组和MSLZ+sh-Wnt1组;除Control组外, 其他组小鼠采用葡聚糖硫酸钠（DSS）构建UC模型。Control组、UC组、MSLZ组小鼠造模结束后每日 腹腔注射5×109 pfu/ml NC-shRNA, MSLZ+sh-Wnt1组小鼠每日腹腔注射5×109 pfu/ml shRNA-Wnt1;MSLZ组和MSLZ+sh-Wnt1组 小鼠每日灌胃400 mg/kg MSLZ, Control、UC组每日灌胃等剂量无菌蒸馏水。检测各组小鼠结肠长度, 试剂盒检测各组小鼠血 清D-乳酸（D-LA）和脂多糖（LPS）含量以及二胺氧化酶（DAO）活性;TUNEL染色检测各组小鼠结肠组织细胞凋亡;免疫组化检 测结肠组织黏蛋白2（MUC2）和上皮细胞间紧密连接蛋白（Occludin）表达;Western blot检测结肠组织Wnt1、β-catenin、B淋巴细 胞瘤-2（Bcl-2）、Bcl-2相关X蛋白（Bax）表达。结果: MSLZ能明显增加UC小鼠结肠长度, 升高结肠组织MUC2、Occludin、Wnt1、 β-catenin和Bcl-2表达, 降低血清D-LA、LPS、DAO水平、结肠组织细胞凋亡率及Bax表达;MSLZ+sh-Wnt1可部分逆转MSLZ对 结肠屏障功能的保护作用（均P<0.05）。结论: MSLZ能抑制UC小鼠结肠黏膜细胞凋亡, 减轻结肠组织病理损伤, 改善结肠黏 膜屏障功能, 可能与激活Wnt1信号有关. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |