التفاصيل البيبلوغرافية
| العنوان: |
Air cleaner prototype: Reduction of airborne viruses and effects of UV-C irradiation on virus concentration and RNA copy numbers considering modeled residence times and doses. |
| المؤلفون: |
Schulz, Jochen1 (AUTHOR) jochen.schulz@tiho-hannover.de, Lochte, Vivian Natascha1 (AUTHOR), Blessing, Sebastian2 (AUTHOR), Stroh, Alexander2 (AUTHOR), Kriegseis, Jochen2 (AUTHOR), Kemper, Nicole1 (AUTHOR), Limbach, Martin3 (AUTHOR), Hahn, Horst3 (AUTHOR) |
| المصدر: |
Aerosol Science & Technology. Jan2025, Vol. 59 Issue 1, p66-78. 13p. |
| مصطلحات موضوعية: |
*AIR flow, *LAMINAR flow, *NUCLEIC acids, *AUTOMATIC timers, *RNA viruses |
| مستخلص: |
A prototype of an air cleaner was investigated for its ability to inactivate airborne MS2 viruses via UV-C irradiation. AGI-30 impingers were used to measure virus concentrations and RNA copy numbers at the air cleaner's inlet and outlet, which was integrated into a testing system. UV-C doses were estimated by modeling the viruses' dwell times in the device and measuring the UV-C radiance level. The appliance reduced infectious viruses by 99% to 92% in airflows ranging from 113 to 153 m³/h, with the reduction rate decreasing as airflow increased. Estimated UV-C doses ranged between 2.11 and 2.95 mWs/cm2. There was a significant correlation between the virus reduction and corresponding doses (Spearman ρ = 0.77, p = 0.0092; Kendall's τ = 0.60, p = 0.0157). Modeling showed that assuming laminar flows or air exchange rates would overestimate the viral particles' residence time in the device. Measurements indicated that viral particles remained partly trapped in the device, and RNA copy numbers did not correlate with the number of infectious viruses. RNA copy number concentrations were up to 3.7 log units above plaque-forming units (PFU) levels (for non-irradiated samples) and reduced only by 19% following UV-C exposure. This discrepancy could be attributed to the RT-PCR used, which also detects RNA fragments from incomplete or noninfectious virus particles. The mismatch between RNA copy detection and the number of infectious viruses raises questions about the appropriateness of using nucleic acid copy numbers for risk assessments or modeling. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |