التفاصيل البيبلوغرافية
| العنوان: |
Positivity Status and Molecular Characterization of Porcine Parvoviruses 1 Through 8 (PPV1-PPV8) from Slaughtered Pigs in China. |
| المؤلفون: |
Zhao, Dashi1 (AUTHOR) 18246986065@163.com, Lin, Hong1,2,3,4,5 (AUTHOR) linhonglynn@yzu.edu.cn, Huang, Zitao6 (AUTHOR) 13450818935@163.com, Zhou, Yajie1 (AUTHOR) zhouyj202405@163.com, Qi, Wenhao1 (AUTHOR) qiwenhao2829@163.com, Cui, Meng1 (AUTHOR) cuimeng201902@163.com, Qiu, Ming1 (AUTHOR) qiuming1997@126.com, Zhu, Jianzhong1,2,4,5 (AUTHOR) jzzhu@yzu.edu.cn, Chen, Nanhua1,2,3,4,5 (AUTHOR) chnhlh@126.com |
| المصدر: |
Animals (2076-2615). Nov2024, Vol. 14 Issue 22, p3238. 11p. |
| مصطلحات موضوعية: |
*ANIMAL herds, *SEQUENCE alignment, *PARVOVIRUSES, *SWINE, *MIXED infections |
| مستخلص: |
Simple Summary: In addition to porcine parvovirus 1 (PPV1), seven new PPVs (PPV2-PPV8) have been identified in the last two decades. However, the prevalence and evolution of PPVs in slaughtered pigs in China are still unclear. The infection status in slaughtered pigs could reflect the overall health situation during pig production in swine herds. Therefore, we detected the infection status of PPVs in 353 samples collected from slaughtered pigs in six regions of China in 2023. Overall, 79.32% of the samples were PPV positive, with 67.50% PPV-positive samples co-infecting with two to six PPVs. Six species of PPVs were detected, except for PPV4 and PPV8. Representative PPV genomes were determined for evaluating evolutionary relationships and detecting recombination events. A genome-based phylogenetic tree confirmed the PCR results, and cross-over events were detected in the PPV2 and PPV3 strains identified in this study. This study provided the first clue on the prevalence and evolution of PPVs in slaughtered pigs in China. Porcine parvoviruses one through eight (PPV1-PPV8) are prevalent in Chinese swine herds. However, the infection status of all these PPVs in slaughtered pigs is still unclarified. In this study, we detected PPV1-PPV8 in 353 tissue samples collected from slaughtered pigs from six regions of China in 2023. At least one species of PPV was detected in 79.32% of the samples (280 out of 353). Six PPV species were detected, except for PPV4 and PPV8, in slaughtered pigs, within which PPV3 (49.86%), PPV2 (42.49%), and PPV7 (42.21%) were predominant, followed by PPV1 (13.31%), PPV6 (13.31%), and PPV5 (8.22%). Noticeably, co-infection was frequently detected, with 67.50% of PPV-positive samples (189 out of 280) co-infecting with two to six PPVs. In addition, one representative genome for each detected PPV was determined. Multiple sequence alignment determined a large number of substitutions in capsid proteins of PPVs. Genome-based phylogenetic analysis confirmed the PCR detection results. Recombination detection identified two potential recombinants (PPV2 GDCZ2023-2088 strain and PPV3 HLJSYS2023-1654 strain) in slaughtered pigs. Overall, this study provides new insights into the prevalence and evolution of PPVs, particularly in slaughtered pigs in China. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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