التفاصيل البيبلوغرافية
| العنوان: |
Metabolic engineering of Halomonas bluephagenesis for the production of ethylene glycol and glycolate from xylose. |
| المؤلفون: |
Liu, Yuzhong1 (AUTHOR), Huo, Kai1 (AUTHOR), Tan, Biwei1 (AUTHOR), He, Xulin1 (AUTHOR), Wu, Qiong2 (AUTHOR), Li, Zheng-Jun1 (AUTHOR) lizj@mail.buct.edu.cn |
| المصدر: |
Journal of Biotechnology. Dec2024, Vol. 396, p36-40. 5p. |
| مصطلحات موضوعية: |
*ETHYLENE glycol, *ALCOHOL dehydrogenase, *XYLOSE, *TITERS, *BOTTLES |
| مستخلص: |
Halophilic Halomonas bluephagenesis , a natural producer of poly-3-hydroxybutyrate (PHB), was metabolically engineered to synthesize ethylene glycol and glycolate from xylose. Xylose utilization was achieved by overexpressing either the xylonate pathway or the ribulose-1-phosphate pathway. The key genes encoding for xylonate dehydratase and 2-keto-3-deoxy-xylonate aldolase in the xylonate pathway were screened. With further overexpressing aldehyde reductase gene yjgB , ethylene glycol accumulation was improved to 0.91 g/L, accompanied with 1.48 g/L of PHB accumulation. The disruption of native glycolate oxidase was found to be essential for glycolate production, and the defective recombinant strain produced 0.80 g/L glycolate with 1.14 g/L PHB in shake flask cultures. These results indicated that H. bluephagenesis has the potential to produce diverse metabolic chemicals from xylose. • H. bluephagenesis was firstly engineered to produce ethylene glycol and glycolate. • Two xylose assimilation pathways were established in H. bluephagenesis. • Ethylene glycol and glycolate titers reached 0.91 g/L and 0.80 g/L in shake flasks, respectively. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |