التفاصيل البيبلوغرافية
| العنوان: |
The dynamics and functional impact of tRNA repertoires during early embryogenesis in zebrafish. |
| المؤلفون: |
Reimão-Pinto, Madalena M1 (AUTHOR) madalena.pinto@unibas.ch, Behrens, Andrew2 (AUTHOR), Forcelloni, Sergio2 (AUTHOR), Fröhlich, Klemens1 (AUTHOR), Kaya, Selay2 (AUTHOR), Nedialkova, Danny D2,3 (AUTHOR) nedialkova@biochem.mpg.de |
| المصدر: |
EMBO Journal. Nov2024, Vol. 43 Issue 22, p5747-5779. 33p. |
| مصطلحات موضوعية: |
*GENE expression, *RNA regulation, *RNA polymerases, *GENETIC transcription, *EMBRYOLOGY, *TRANSFER RNA, *GASTRULATION, *GENETIC translation |
| مستخلص: |
Embryogenesis entails dramatic shifts in mRNA translation and turnover that reprogram gene expression during cellular proliferation and differentiation. Codon identity modulates mRNA stability during early vertebrate embryogenesis, but how the composition of tRNA pools is matched to translational demand is unknown. By quantitative profiling of tRNA repertoires in zebrafish embryos during the maternal-to-zygotic transition, we show that zygotic tRNA repertoires are established after the onset of gastrulation, succeeding the major wave of zygotic mRNA transcription. Maternal and zygotic tRNA pools are distinct, but their reprogramming does not result in a better match to the codon content of the zygotic transcriptome. Instead, we find that an increase in global translation at gastrulation sensitizes decoding rates to tRNA supply, thus destabilizing maternal mRNAs enriched in slowly translated codons. Translational activation and zygotic tRNA expression temporally coincide with an increase of TORC1 activity at gastrulation, which phosphorylates and inactivates the RNA polymerase III repressor Maf1a/b. Our data indicate that a switch in global translation, rather than tRNA reprogramming, determines the onset of codon-dependent maternal mRNA decay during zebrafish embryogenesis. Synopsis: Embryonic development requires a coordinate and precise regulation of mRNA translation and stability. This study reveals the composition of tRNA repertoires during the maternal-to-zygotic transition in zebrafish and their impact on mRNA decoding speed and codon-dependent maternal mRNA stability. Zebrafish tRNA profiles are re-shaped as embryos enter gastrulation. Zygotic tRNA expression does not render tRNA pools more suited to global codon demand. Zygotic tRNA expression onset temporally coincides with increased TORC1 activity and Maf1 phosphorylation. An increase in global translation at gastrulation sensitizes maternal mRNA decoding rates to tRNA supply. Increased translational activity during the maternal-to-zygotic transition sensitizes maternal mRNA decoding rates to tRNA supply. [ABSTRACT FROM AUTHOR] |
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