Academic Journal
Dendritic/antigen presenting cell mediated provision of T-cell receptor gamma delta (TCRγδ) expressing cells contributes to improving antileukemic reactions ex vivo.
| العنوان: | Dendritic/antigen presenting cell mediated provision of T-cell receptor gamma delta (TCRγδ) expressing cells contributes to improving antileukemic reactions ex vivo. |
|---|---|
| المؤلفون: | Rackl, Elias1 (AUTHOR) elias.rackl@hotmail.de, Hartz, Anne1 (AUTHOR) as.hartz@t-online.de, Aslan Rejeski, Hazal1 (AUTHOR) hazlaslan@gmail.com, Li, Lin1 (AUTHOR) lilin199209106666@gmail.com, Klauer, Lara Kristina1 (AUTHOR) lara.klauer@kabelmail.de, Ugur, Selda1 (AUTHOR) seldaugur94@hotmail.de, Pepeldjiyska, Elena1 (AUTHOR) elena.pepeldjiyska@gmail.com, Amend, Carina1 (AUTHOR) carina_gunsilius@web.de, Weinmann, Melanie1 (AUTHOR) melli.weinmann@hotmail.de, Doraneh-Gard, Fatemeh1 (AUTHOR) nikoo_792000@yahoo.com, Stein, Julian1 (AUTHOR) julian.stein@med.uni-muenchen.de, Reiter, Nina1 (AUTHOR) nina.d.reiter@web.de, Seidel, Corinna L.2 (AUTHOR) corinna.seidel@uk-erlangen.de, Plett, Caroline1 (AUTHOR) caroline.plett@googlemail.com, Amberger, Daniel Christoph1 (AUTHOR) daniel.amberger@gmx.at, Bojko, Peter3 (AUTHOR) peter.bojko@swmbrk.de, Kraemer, Doris4 (AUTHOR) kraemerd@kkh-hagen.de, Schmohl, Jörg5 (AUTHOR) joerg.schmohl@diak-stuttgart.de, Rank, Andreas6,7 (AUTHOR) andreas.rank@uk-augsburg.de, Schmid, Christoph6,7 (AUTHOR) christoph.schmid@uk-augsburg.de |
| المصدر: | Molecular Immunology. Nov2024, Vol. 175, p40-54. 15p. |
| مصطلحات موضوعية: | *ANTIGEN presenting cells, *GRANULOCYTE-macrophage colony-stimulating factor, *T cell receptors, *PROSTAGLANDIN E1, *DENDRITIC cells |
| مستخلص: | T-cell receptor gamma delta (TCRγδ) expressing T-cells are known to mediate an MHC-independent immune response and could therefore qualify for immune therapies. We examined the influence of dendritic cells(DC)/antigen presenting cell (APC) generated from blast-containing whole blood (WB) samples from AML and MDS patients on the provision of (leukemia-specific) TCRγδ expressing T-cells after mixed lymphocyte culture (MLC). Kit-M (granulocyte-macrophage colony-stimulating factor (GM-CSF) + prostaglandin E1 (PGE1)) or Kit-I (GM-CSF + Picibanil) were used to generate leukemia derived APC/DC (DC leu)from WB, which were subsequently used to stimulate T-cell enriched MLC. Immune cell composition and functionality were analysed using degranulation- (DEG), intracellular cytokine- (INTCYT) and cytotoxicity fluorolysis- (CTX) assays. Flow cytometry was used for cell quantification. We found increased frequencies of APCs/DCs and their subtypes after Kit-treatment of healthy and patients´ WB compared to control, as well as an increased stimulation and activation of several types of immune reactive cells after MLC. Higher frequencies of TCRγδ expressing leukemia-specific degranulation and intracellularly cytokine producing T-cells were found. The effect of Kit-M-treatment on frequencies of TCRγδ expressing cells and their degranulation could be correlated with the Kit-M-mediated blast lysis compared to control. We also found higher frequencies of TCRγδ expressing T-cells in AML patients´ samples with an achieved remission (compared to blast persistence) after induction chemotherapy. This might point to APC/DC-mediated effects resulting in the provision of leukemia-specific TCRγδ expressing T-cells: Moreover a quantification of TCRγδ expressing T-cells might contribute to predict prognosis of AML/MDS patients. • leukemia derived APC/leukemia derived dendritic cells induce TCRγδ expressing cells. • blastolytic potential ex vivo of TCRγδ expressing cells. • TCRγδ might be useful as a prognostic marker. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: | Academic Search Index |
| تدمد: | 01615890 |
|---|---|
| DOI: | 10.1016/j.molimm.2024.09.007 |