التفاصيل البيبلوغرافية
| العنوان: |
Development of a blocking ELISA for detection of Japanese encephalitis virus antibodies in pig and horse sera. |
| المؤلفون: |
Dong-Kun Yang1 yangdk@korea.kr, Eun-Ju Kim1, Sang Ho Jang2, Hye Jung Lee1, Bitna Kim2, Jin A Lee2, Ju-Yeon Lee1, Yun Sang Cho1 |
| المصدر: |
Korean Journal of Veterinary Research. Sep2024, Vol. 64 Issue 3, p1-9. 9p. |
| مصطلحات موضوعية: |
*JAPANESE encephalitis viruses, *VIRAL antibodies, *ENZYME-linked immunosorbent assay, *HORSERADISH peroxidase, *ANIMAL species, *SWINE |
| مستخلص: |
Japanese encephalitis virus (JEV) is a mosquito-borne virus that can infect pigs, horses, and other mammals, including humans. Sero-epidemiological investigations of JEV have been performed using hemagglutination inhibition (HI), virus neutralization (VN) tests and enzyme-linked immunosorbent assay (ELISA). A need exists for a new ELISA that can detect JEV antibodies in the sera of several animal species. We aimed to develop a blocking ELISA (B-ELISA) for detecting JEV antibodies in pig and horse serum samples. JEV antibodies in 218 pig and 315 horse serum samples were measured using HI and VN tests. The purified KV1899-306 strain was used as an antigen for B-ELISA. The purified antibody (7A13) was conjugated with horseradish peroxidase and used as a detector antibody. The sera of pigs and horses to measure antibody against JEV were subjected to B-ELISA and analyzed. The B-ELISA had a diagnostic sensitivity of 94.6% to 100%, a specificity of 91.2 to 100%, and an accuracy of 94.9 to 98.6% compared with those of the HI and VN tests in pig and horse sera. The B-ELISA had a higher correlation with pig sera (r = 0.89 and 0.90 for VN and HI) than with horse sera (r = 0.75 and to 0.79). The new B-ELISA could be useful in the sero-surveillance of JEV in pig and horse sera and replace indirect ELISA. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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