التفاصيل البيبلوغرافية
| العنوان: |
The Application of Duck Embryonic Fibroblasts CCL-141 as a Cell Model for Adipogenesis. |
| المؤلفون: |
Sun, Dan-Dan1 (AUTHOR) sundandan@cau.edu.cn, Li, Xiao-Qin1 (AUTHOR), Liu, Yong-Tong1 (AUTHOR), Ge, Meng-Qi1 (AUTHOR), Hou, Zhuo-Cheng1 (AUTHOR) zchou@cau.edu.cn |
| المصدر: |
Animals (2076-2615). Oct2024, Vol. 14 Issue 20, p2973. 14p. |
| مصطلحات موضوعية: |
*STAINS & staining (Microscopy), *FAT cells, *GENE knockout, *GENE expression, *TRETINOIN, *ADIPOGENESIS |
| مستخلص: |
Simple Summary: This study demonstrates that the duck embryo fibroblast cell line CCL-141 can undergo adipogenesis, vital for understanding fat cell development in ducks. Treatments with chicken serum, fatty acids, insulin, and all-trans retinoic acid induced fat cell formation, as evidenced by Oil Red O staining and a gene expression analysis. Moreover, the CRISPR/Cas9 knockout of the adipogenesis gene PPARγ in CCL-141 cells confirmed the cell line's utility for studying adipogenesis-related gene functions. These findings validate CCL-141 as a model for adipogenesis research, which will aid in uncovering its regulatory mechanisms. The duck embryo fibroblast cell line CCL-141, which is currently the only commercialized duck cell line, has been underexplored in adipogenesis research. (1) Background: This study establishes an experimental protocol to induce adipogenesis in CCL-141 cells, addressing the importance of understanding gene functions in this process. (2) Methods: Chicken serum, fatty acids, insulin, and all-trans retinoic acid were used to treat CCL-141 cells, with adipogenesis confirmed by Oil Red O staining and gene expression quantification. CRISPR/Cas9 technology was applied to knockout PPARγ, and the resulting adipogenic phenotype was assessed. (3) Results: The treatments promoted adipogenesis, and the knockout of PPARγ validated the cell line's utility for gene function studies. (4) Conclusions: CCL-141 cells are a suitable model for investigating duck adipogenesis, contributing to the understanding of regulatory factors in this biological process. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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