التفاصيل البيبلوغرافية
| العنوان: |
DETECTION OF FASCIOLA HEPATICA USING NESTED-PCR IN THE SLAUGHTERHOUSES OF ALBORZ, IRAN. |
| المؤلفون: |
KALVANI, A. REZAEE1 arezaiy330@gmail.com, KHIAV, L. ABDOLMOHAMMADI1, HOSSEINI, S. R.2 |
| المصدر: |
Bulgarian Journal of Veterinary Medicine. Dec2024, Vol. 27 Issue 4, p639-645. 7p. |
| مصطلحات موضوعية: |
*FASCIOLA hepatica, *GENE amplification, *PARASITIC diseases, *FASCIOLIASIS, *SLAUGHTERING |
| مستخلص: |
Fasciola hepatica is a parasitic liver trematode that causes fasciolosis in humans and dairy animals. Traditional detection of infection is based on a microscopic examination with low sensitivity. Therefore, this study was carried out to develop an accurate and rapid method to detect F. hepatica in Alborz province. In this cross-sectional study, 386 samples were collected from livers of cattle and sheep in three slaughterhouses of Alborz. DNA was extracted, and nested-PCR was carried out based on the F. hepatica ITS-2 sequence. DNA amplification showed first and second PCR products with expected size of 336 and 208 bp respectively, as unique for F. hepatica. The results showed no cross-reaction with the negative control. Thirty-five liver samples were positive for this parasitic infection. Totally, the prevalence of F. hepatica in the slaughterhouses of Alborz, Iran was 9.07%. This is the first study of the molecular detection of F. hepatica using nested-PCR in Alborz. It is a sensitive and rapid method that will help evaluate the prevalence rate of F. hepatica infection. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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